CAJ | 학술논문

目的探讨外源性晚期氧化蛋白产物(advanced oxidation protein products,AOPPs)关节腔内注射对兔骨关节炎模型中关节软骨的组织形态学及滑膜中基质金属蛋白酶(matrix metalloproteinases,MMPs)表达的影响.方法将48只雄性新两兰大白兔随机平均分为AOPPs组、磷酸盐缓冲液组和假手术组,每组16只.其中假手术组仅显露前十字韧带后缝合切口;AOPPs组、磷酸盐缓冲液组采用前十字韧带离断加内侧半月板切除术建立骨关节炎模型,而后给予不同的干预.AOPPs组于双侧膝关节内注射浓度为200 μg/ml的AOPPs 1 ml,隔日一次;磷酸盐缓冲液组注射1 ml磷酸盐缓冲液,隔日一次.三组均于4、8周后各处死8只并取膝关节标本.结果 AOPPs组4周和8周India ink染色大体评分分别为(4.19±0.60)分、(5.75±0.60)分,假手术组分别为(1.06±0.18)分、(1.38±0.60)分,磷酸盐缓冲液组分别为(2.50±0.46)分、(3.06±0.62)分.组织学观察AOPPs组4周和8周Mankin评分分别为(8.19±0.70)分、(11.94±0.90)分,似手术组分别为(0.75±0.53)分、(1.06±0.73)分,磷酸盐缓冲液组分别为(4.25±1.46)分、(4.50±0.89)分.AOPPs组4周和8周滑膜组织中MMP-3相对表达量分别为1.006±0.080、1.098±0.088,假手术组分别为0.065±0.006、0.053±0.011,磷酸盐缓冲液组分别为0.552±0.024、0.839±0.084.AOPPs组4周和8周滑膜组织中MMP-13的表达量分别为0.966±0.080、1.621±0.041,假手术组分别为0.101±0.022、0.367±0.033,磷酸盐缓冲液组分别为0.564±0.030、1.322±0.085.以上所有指标检测,三组之间比较差异均有统计学意义.结论外源性AOPPs关节腔内注射,可使滑膜细胞MMP-3、MMP-13表达上调,加速软骨退变,进而推进骨关节炎的发展.
목적 탐토외원성만기양화단백산물(advanced oxidation protein products,AOPPs)관절강내주사대토골관절염모형중관절연골적조직형태학급활막중기질금속단백매(matrix metalloproteinases,MMPs)표체적영향.방법 장48지웅성신량란대백토수궤평균분위AOPPs조、린산염완충액조화가수술조,매조16지.기중가수술조부현로전십자인대후봉합절구;AOPPs조、린산염완충액조채용전십자인대리단가내측반월판절제술건립골관절염모형,이후급여불동적간예.AOPPs조우쌍측슬관절내주사농도위200 μg/ml적AOPPs 1 ml,격일일차;린산염완충액조주사1 ml린산염완충액,격일일차.삼조균우4、8주후각처사8지병취슬관절표본.결과 AOPPs조4주화8주India ink염색대체평분분별위(4.19±0.60)분、(5.75±0.60)분,가수술조분별위(1.06±0.18)분、(1.38±0.60)분,린산염완충액조분별위(2.50±0.46)분、(3.06±0.62)분.조직학관찰AOPPs조4주화8주Mankin평분분별위(8.19±0.70)분、(11.94±0.90)분,사수술조분별위(0.75±0.53)분、(1.06±0.73)분,린산염완충액조분별위(4.25±1.46)분、(4.50±0.89)분.AOPPs조4주화8주활막조직중MMP-3상대표체량분별위1.006±0.080、1.098±0.088,가수술조분별위0.065±0.006、0.053±0.011,린산염완충액조분별위0.552±0.024、0.839±0.084.AOPPs조4주화8주활막조직중MMP-13적표체량분별위0.966±0.080、1.621±0.041,가수술조분별위0.101±0.022、0.367±0.033,린산염완충액조분별위0.564±0.030、1.322±0.085.이상소유지표검측,삼조지간비교차이균유통계학의의.결론 외원성AOPPs관절강내주사,가사활막세포MMP-3、MMP-13표체상조,가속연골퇴변,진이추진골관절염적발전.
Objective To observe the effect of advanced oxidation protein products (AOPPs) on articular and synovial in a rabbit model of osteoarthritis (OA).Methods 48 male New Zealand rabbits were randomly divided into 3 groups:AOPPs group,PBS group and sham-operated group.OA model were created in AOPPs group and PBS group by anterior cruciate ligament transection and medial meniscus resection (ACLT+MMx).then intra-articular injection of 1 ml AOPPs or PBS were performed once every other day in AOPPs group and PBS group,respectively.In sham-operated group,the anterior cruciate ligament was just exposed without transection,and then the incision was sutured.All rabbits were saerificed after 4 and 8 weeks of intervention,respectively.Results The India ink seore of 4 and 8 weeks were 4.19±0.60,5.75±0.60 in AOPPs group,and 1.06±0.18,1.38±0.60 in sham-operated group,2.50±0.46、3.06±0.62 in PBS group,respectively.In addition,the differences were statistically significant among the three groups.The Mankin score of 4 and 8 weeks were 8.19±0.70,11.94±0.90 in AOPPs group,and 0.75±0.53,1.06± 0.73 in sham-operated group,4.25± 1.46、4.50±0.89 in PBS group,respectively.The differences were statistically significant among the three groups.Meanwhile,the protein expression level of matrix metalloproteinases (MMP)-3 on synovial at 4 and 8 weeks in AOPPs group were 1.006±0.080,1.098±0.088;0.065±0.006,0.053±0.011 in sham-operated group;and 0.552±0.024,0.839±0.084 in PBS group,respectively.The proteiu expression level of MMP-13 on synovial at 4 and 8 weeks in AOPPs group were 0.966±0.080,1.621 ±0.041;0.101±0.022,0.367±0.033 in sham-operated group;and 0.564±0.030,1.322±0.085 in PBS group,respectively.The differences were statistically significant among the three groups at two times.Conclusion AOPPs participate in the occurrence and development of artieular cartilage by upregulating the protein expression of MMP-3 and MMP-13 on synovial.