贵州农业科学
貴州農業科學
귀주농업과학
GUIZHOU AGRICULTURAL SCIENCES
2015年
6期
77-79,82
,共4页
印楝%印楝素%悬浮细胞%接种量%通气比
印楝%印楝素%懸浮細胞%接種量%通氣比
인련%인련소%현부세포%접충량%통기비
Azadirachta indica%azadirachin%suspension cell%inoculum size%compression/ventilation ratio
为印楝悬浮细胞扩大培养提供依据，利用5 L 气升式发酵罐，以印楝悬浮细胞作种子细胞，研究接种量与通气比对印楝悬浮细胞生长和印楝素产量的影响。结果表明：在反应器中，印楝悬浮细胞生长和印楝素产量呈偶联型；种子细胞适宜接种量和通气比分别为60 g／L 和0．2 vvm，在此条件下，悬浮培养基质中的 pH 呈先降后升的变化趋势，细胞干重和印楝素产量分别为11．41 g DW／L 和8．32 mg／g，添加复合诱导子后48 h 印楝素产量最大，达94．78 mg／L。
為印楝懸浮細胞擴大培養提供依據，利用5 L 氣升式髮酵罐，以印楝懸浮細胞作種子細胞，研究接種量與通氣比對印楝懸浮細胞生長和印楝素產量的影響。結果錶明：在反應器中，印楝懸浮細胞生長和印楝素產量呈偶聯型；種子細胞適宜接種量和通氣比分彆為60 g／L 和0．2 vvm，在此條件下，懸浮培養基質中的 pH 呈先降後升的變化趨勢，細胞榦重和印楝素產量分彆為11．41 g DW／L 和8．32 mg／g，添加複閤誘導子後48 h 印楝素產量最大，達94．78 mg／L。
위인련현부세포확대배양제공의거，이용5 L 기승식발효관，이인련현부세포작충자세포，연구접충량여통기비대인련현부세포생장화인련소산량적영향。결과표명：재반응기중，인련현부세포생장화인련소산량정우련형；충자세포괄의접충량화통기비분별위60 g／L 화0．2 vvm，재차조건하，현부배양기질중적 pH 정선강후승적변화추세，세포간중화인련소산량분별위11．41 g DW／L 화8．32 mg／g，첨가복합유도자후48 h 인련소산량최대，체94．78 mg／L。
The effects of inoculum size and compression/ventilation ratio on growth of A.indica suspension cells and A.indica yield were analyzed when A.indica suspension cells was cultured in 5 L airilift ferment reactor to provide a reference for enlarging culture of A.indica suspension cells.The results showed that growth of A.indica suspension cells and A.indica yield in the airilift ferment reactor represent a coupled type.The optimum inoculum size and compression/ventilation ratio for A.indica suspension cells is 60 g/L and 0.2 vvm respectively.The cell dry weight and azadirachin yield are 11.41 g DW/L and 8.32 mg/g under the optimum culture conditions respectively.Adding compound inductors can improve azadirachin yield significantly and the azadirachin yield reaches 94.78 mg/L after 48 h.
