中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
24期
1957-1960
,共4页
哮喘%激素抵抗%白杨素%小鼠
哮喘%激素牴抗%白楊素%小鼠
효천%격소저항%백양소%소서
Asthma%Steroid resistance%Chrysin%Mice
目的 探讨白杨素对激素抵抗型支气管哮喘小鼠模型的干预作用.方法 利用卵清蛋白致敏和激发小鼠,建立支气管哮喘模型,利用脂多糖诱导激素抵抗.48只雌性BALB/c小鼠按随机数字表法随机分为对照组(A组)、卵清蛋白组(B组)、脂多糖+卵清蛋白组(C组)、脂多糖+卵清蛋白+地塞米松组(D组)、脂多糖+卵清蛋白+白杨素组(E组)和脂多糖+卵清蛋白+地塞米松+白杨素组(F组)各8只.在第1、14天,A组腹腔注射磷酸盐缓冲液,B、C、D、E、F组腹腔注射卵清蛋白(20 μg)/氢氧化铝混合液致敏;在第27天,A、B组进行磷酸盐缓冲液滴鼻,C、D、E、F组进行脂多糖(10 μg)滴鼻.在第28、29、30天,A组以磷酸盐缓冲液进行雾化30 min,B、C、D、E、F组以1％卵清蛋白磷酸盐缓冲液雾化30 min激发.D组在雾化前一天腹腔注射磷酸盐缓冲液,每次雾化前30 min腹腔注射地塞米松(3 mg/kg);E组雾化前一天及每次雾化前lh腹腔注射白杨素(50 mg/kg),F组为雾化前一天及每次雾化前1 h腹腔注射白杨素(50 mg/kg)、每次雾化前30 min腹腔注射地塞米松(3 mg/kg).A、B、C组参照上述方法在雾化前一天及每次雾化前腹腔注射磷酸盐缓冲液.分别利用HE和PAS染色比较小鼠肺组织病理改变,显微镜下计数支气管肺泡灌洗液(BALF)中白细胞总数,酶联免疫吸附(ELISA)法测定BALF中白细胞介素(IL)-4、IL-13及血清中lgE水平.结果 F组小鼠肺组织炎症损伤较C组明显减轻;D、E、F组气道杯状细胞占上皮细胞百分比均显著低于C组[(54.5±6.9)％、(53.3±8.2)％、(23.8±7.0)％比(71.3±12.2)％,均P＜0.01];E、F组BALF中白细胞总数均显著低于C组[(1.22±0.23)×104、(0.98±0.25)×104比(2.56±0.18)×104个/ml,均P＜0.01];D、F组IL-4水平均显著低于C组[(118±7)、(124±5)比(138±6) pg/ml,均P＜0.01];E、F组IL-13水平均显著低于C组[(787±57)、(484±32)比(1 121±132) pg/ml,均P＜0.01];D、E、F组血清中IgE水平均显著低于C组[(10 310±494)、(10 771±650)、(7 529±485)比(12 618±595) ng/ml,均P＜0.01].结论 白杨素能够恢复激素抵抗型支气管哮喘小鼠对激素治疗的敏感性.
目的 探討白楊素對激素牴抗型支氣管哮喘小鼠模型的榦預作用.方法 利用卵清蛋白緻敏和激髮小鼠,建立支氣管哮喘模型,利用脂多糖誘導激素牴抗.48隻雌性BALB/c小鼠按隨機數字錶法隨機分為對照組(A組)、卵清蛋白組(B組)、脂多糖+卵清蛋白組(C組)、脂多糖+卵清蛋白+地塞米鬆組(D組)、脂多糖+卵清蛋白+白楊素組(E組)和脂多糖+卵清蛋白+地塞米鬆+白楊素組(F組)各8隻.在第1、14天,A組腹腔註射燐痠鹽緩遲液,B、C、D、E、F組腹腔註射卵清蛋白(20 μg)/氫氧化鋁混閤液緻敏;在第27天,A、B組進行燐痠鹽緩遲液滴鼻,C、D、E、F組進行脂多糖(10 μg)滴鼻.在第28、29、30天,A組以燐痠鹽緩遲液進行霧化30 min,B、C、D、E、F組以1％卵清蛋白燐痠鹽緩遲液霧化30 min激髮.D組在霧化前一天腹腔註射燐痠鹽緩遲液,每次霧化前30 min腹腔註射地塞米鬆(3 mg/kg);E組霧化前一天及每次霧化前lh腹腔註射白楊素(50 mg/kg),F組為霧化前一天及每次霧化前1 h腹腔註射白楊素(50 mg/kg)、每次霧化前30 min腹腔註射地塞米鬆(3 mg/kg).A、B、C組參照上述方法在霧化前一天及每次霧化前腹腔註射燐痠鹽緩遲液.分彆利用HE和PAS染色比較小鼠肺組織病理改變,顯微鏡下計數支氣管肺泡灌洗液(BALF)中白細胞總數,酶聯免疫吸附(ELISA)法測定BALF中白細胞介素(IL)-4、IL-13及血清中lgE水平.結果 F組小鼠肺組織炎癥損傷較C組明顯減輕;D、E、F組氣道杯狀細胞佔上皮細胞百分比均顯著低于C組[(54.5±6.9)％、(53.3±8.2)％、(23.8±7.0)％比(71.3±12.2)％,均P＜0.01];E、F組BALF中白細胞總數均顯著低于C組[(1.22±0.23)×104、(0.98±0.25)×104比(2.56±0.18)×104箇/ml,均P＜0.01];D、F組IL-4水平均顯著低于C組[(118±7)、(124±5)比(138±6) pg/ml,均P＜0.01];E、F組IL-13水平均顯著低于C組[(787±57)、(484±32)比(1 121±132) pg/ml,均P＜0.01];D、E、F組血清中IgE水平均顯著低于C組[(10 310±494)、(10 771±650)、(7 529±485)比(12 618±595) ng/ml,均P＜0.01].結論 白楊素能夠恢複激素牴抗型支氣管哮喘小鼠對激素治療的敏感性.
목적 탐토백양소대격소저항형지기관효천소서모형적간예작용.방법 이용란청단백치민화격발소서,건립지기관효천모형,이용지다당유도격소저항.48지자성BALB/c소서안수궤수자표법수궤분위대조조(A조)、란청단백조(B조)、지다당+란청단백조(C조)、지다당+란청단백+지새미송조(D조)、지다당+란청단백+백양소조(E조)화지다당+란청단백+지새미송+백양소조(F조)각8지.재제1、14천,A조복강주사린산염완충액,B、C、D、E、F조복강주사란청단백(20 μg)/경양화려혼합액치민;재제27천,A、B조진행린산염완충액적비,C、D、E、F조진행지다당(10 μg)적비.재제28、29、30천,A조이린산염완충액진행무화30 min,B、C、D、E、F조이1％란청단백린산염완충액무화30 min격발.D조재무화전일천복강주사린산염완충액,매차무화전30 min복강주사지새미송(3 mg/kg);E조무화전일천급매차무화전lh복강주사백양소(50 mg/kg),F조위무화전일천급매차무화전1 h복강주사백양소(50 mg/kg)、매차무화전30 min복강주사지새미송(3 mg/kg).A、B、C조삼조상술방법재무화전일천급매차무화전복강주사린산염완충액.분별이용HE화PAS염색비교소서폐조직병리개변,현미경하계수지기관폐포관세액(BALF)중백세포총수,매련면역흡부(ELISA)법측정BALF중백세포개소(IL)-4、IL-13급혈청중lgE수평.결과 F조소서폐조직염증손상교C조명현감경;D、E、F조기도배상세포점상피세포백분비균현저저우C조[(54.5±6.9)％、(53.3±8.2)％、(23.8±7.0)％비(71.3±12.2)％,균P＜0.01];E、F조BALF중백세포총수균현저저우C조[(1.22±0.23)×104、(0.98±0.25)×104비(2.56±0.18)×104개/ml,균P＜0.01];D、F조IL-4수평균현저저우C조[(118±7)、(124±5)비(138±6) pg/ml,균P＜0.01];E、F조IL-13수평균현저저우C조[(787±57)、(484±32)비(1 121±132) pg/ml,균P＜0.01];D、E、F조혈청중IgE수평균현저저우C조[(10 310±494)、(10 771±650)、(7 529±485)비(12 618±595) ng/ml,균P＜0.01].결론 백양소능구회복격소저항형지기관효천소서대격소치료적민감성.
Objective To explore the therapeutic effects of chrysin for steroid-resistant asthma in a murine model.Methods Lipopolysaccharide (LPS) was used to induce steroid-resistant asthma in a murine model sensitized and challenged with ovalbumin (OVA).Forty-eight female BALB/c mice were randomly divided into 6 groups of control (A),OVA (B),LPS + OVA (C),LPS + OVA + dexamethasone (D),LPS + OVA + chrysin (E) and LPS + OVA + dexamethasone + chrysin (F) (n =8 each).At Day 1 and 14,group A received an intraperitoneal injection of phosphate buffered saline (PBS);groups B,C,D,E and F had an intraperitoneal injection of a mixture of OVA (20 μg) and aluminum hydroxide for sensitization.At Day 27,groups A and B were intranasally instilled with PBS while groups C,D,E and F had an intranasal instillation of LPS (10 μg).At Days 28,29 and 30,groups B,C,D,E and F were challeuged via airway with 1％ OVA in PBS for 30 min and group A with PBS.Group D was intraperitoneally injected with dexamethasone (3 mg/kg) 30 min pre-challenge and PBS one day pre-challenge;group E received an intraperitoneal injection of chrysin (50 mg/kg) at one day and 1 h pre-challenge;group F received dexamethasone (3 mg/kg) 30 min pre-challenge and chrysin (50 mg/kg) at one day and 1 h prechallenge;groups A,B and C had PBS as above.Hematoxylin-eosin (HE) staining and periodic acid-Schiff (PAS) staining of lung tissues were performed to observe the pathologic changes.The total cells in bronchoalveolar lavage fluid (BALF) were counted under microscope.And enzyme-linked immunosorbent assay (ELISA) was applied for detecting interleukin-4 (IL-4) and interleukin-13 (IL-13) in BALF and IgE in sera.Results The inflammatory infiltration in lung tissue of group F was obviously suppressed compared with that of group C.The numbers of PAS-positive cells per bronchus divided by the total number of epithelial cells in group D,E,F were markedly lower than that in group C ((54.5 ± 6.9) ％,(53.3 ±8.2)％,(23.8 ±7.0)％ vs (71.3±12.2)％,allP＜0.01).The total cells in BALF ofgroupE and F significantly decreased versus that of group C ((1.22 ± 0.23) × 104/ml,(0.98 ± 0.25) × 104/ml vs (2.56 ±0.18) × 104/ml,both P ＜0.01).The levels of IL-4 in group D and F were significantly less than that of group C ((118 ± 7),(124 ± 5) vs (138 ± 6) pg/ml,both P ＜ 0.01).The levels of IL-13 in BALF of group E and F significantly decreased compared with that of group C ((787 ±57),(484 ±32) vs (1 121 ± 132) pg/ml,both P ＜0.01).The levels of IgE in sera of group D,E,F were significantly lower those of group C ((10 310 ±494),(10 771 ±650),(7 529 ±485) vs (12 618 ±595) ng/ml,all P ＜0.01).Conclusion Chrysin could improve the therapeutic efficacies of glucocorticoid for steroid-resistant asthma.
