CAJ | 학술논문

为构建优良杂交玉米新品种金玉818的指纹图谱，筛选适用于该品种真实性和纯度鉴定的SSR 引物，利用 SSR 分子标记技术构建指纹图谱，筛选引物对金玉818种子进行纯度鉴定，并对鉴定结果进行验证。结果表明：在40对标准 SSR 引物中筛选出25对多态性好、清晰稳定的引物构建金玉818指纹图谱，筛选出15对适用于该品种真实性和纯度鉴定的引物，其中最佳引物是 bnlg16lk8、umc1125y3、umc1429y7、phi053k2、umc1147y4和 bnlg2305k4；利用 phi053k2、bnlg16lk8和 umc1429y7鉴定金玉818种子的纯度分别为92．5％、92％和93．5％，引物间鉴定结果吻合度高，结果可靠。
위구건우량잡교옥미신품충금옥818적지문도보，사선괄용우해품충진실성화순도감정적SSR 인물，이용 SSR 분자표기기술구건지문도보，사선인물대금옥818충자진행순도감정，병대감정결과진행험증。결과표명：재40대표준 SSR 인물중사선출25대다태성호、청석은정적인물구건금옥818지문도보，사선출15대괄용우해품충진실성화순도감정적인물，기중최가인물시 bnlg16lk8、umc1125y3、umc1429y7、phi053k2、umc1147y4화 bnlg2305k4；이용 phi053k2、bnlg16lk8화 umc1429y7감정금옥818충자적순도분별위92．5％、92％화93．5％，인물간감정결과문합도고，결과가고。
To build fingerprint of the elite corn hybrid Jinyu818,and to screen out the SSR primers suitable for the authenticity and purity identification of the variety,Genomic DNA of Jinyu818 seed was extracted for screening SSR markers that could be applied in fingerprint establishment and purity identification from 40 pairs of SSR primers.Results:25 pairs of primers,which presented stable,clear and polymorphic amplification profiles,were determined as candidates for fingerprint establishment. Moreover,15 pairs of SSR primers were selected as candidates for authenticity and purity identification of Jinyu818. The best primers are bnlg16lk8, umc1125y3, umc1429y7, phi053k2, umc1147y4 and bnlg2305k4.By choosing primers phi053k2、bnlg16lk8 and umc1429y7 to test the purity of Jinyu818 seed, the purity was 92.5%,92% and 93.5%,respectively.With the highly consistency of 3 pairs of primers tests results,it was demonstrated that the results were reliable.