食品与药品
食品與藥品
식품여약품
FOOD AND DRUG
2015年
3期
170-174
,共5页
凌娜%李红秀%仲星%王帆
凌娜%李紅秀%仲星%王帆
릉나%리홍수%중성%왕범
小球藻%铁%营养%生理特性
小毬藻%鐵%營養%生理特性
소구조%철%영양%생리특성
Chlorella pyrenoidosa%iron%nutrient%physiological property
目的：通过向培养液中添加不同浓度的FeSO4和FeCl3，研究不同价态铁及添加方式对小球藻的营养和生理特性的影响。方法用不同浓度的FeSO4和FeCl3培养小球藻，检测小球藻生物量、叶绿素、β-胡萝卜素、蛋白质及可溶性糖含量的变化。结果当FeSO4和FeCl3的浓度分别为12.5μmol/L和25μmol/L时，小球藻的生长最旺盛，生物量、铁富集量、叶绿素、β-胡萝卜素、蛋白质及多糖含量最高。当铁浓度升高至100μmol/L以上时，藻的生长及各项生理指标都明显低于对照组，同时出现藻液变黄甚至藻体死亡现象。另外，分批次添加与一次性添加相比，小球藻生物量、铁富集量、蛋白质和多糖含量均有提高。结论铁的不同价态对小球藻的生长影响有明显差异，Fe2+较之Fe3+表现出更强的藻利用效果，同样浓度下，藻的增殖速度更快，营养品质更高。
目的：通過嚮培養液中添加不同濃度的FeSO4和FeCl3，研究不同價態鐵及添加方式對小毬藻的營養和生理特性的影響。方法用不同濃度的FeSO4和FeCl3培養小毬藻，檢測小毬藻生物量、葉綠素、β-鬍蘿蔔素、蛋白質及可溶性糖含量的變化。結果噹FeSO4和FeCl3的濃度分彆為12.5μmol/L和25μmol/L時，小毬藻的生長最旺盛，生物量、鐵富集量、葉綠素、β-鬍蘿蔔素、蛋白質及多糖含量最高。噹鐵濃度升高至100μmol/L以上時，藻的生長及各項生理指標都明顯低于對照組，同時齣現藻液變黃甚至藻體死亡現象。另外，分批次添加與一次性添加相比，小毬藻生物量、鐵富集量、蛋白質和多糖含量均有提高。結論鐵的不同價態對小毬藻的生長影響有明顯差異，Fe2+較之Fe3+錶現齣更彊的藻利用效果，同樣濃度下，藻的增殖速度更快，營養品質更高。
목적：통과향배양액중첨가불동농도적FeSO4화FeCl3，연구불동개태철급첨가방식대소구조적영양화생리특성적영향。방법용불동농도적FeSO4화FeCl3배양소구조，검측소구조생물량、협록소、β-호라복소、단백질급가용성당함량적변화。결과당FeSO4화FeCl3적농도분별위12.5μmol/L화25μmol/L시，소구조적생장최왕성，생물량、철부집량、협록소、β-호라복소、단백질급다당함량최고。당철농도승고지100μmol/L이상시，조적생장급각항생리지표도명현저우대조조，동시출현조액변황심지조체사망현상。령외，분비차첨가여일차성첨가상비，소구조생물량、철부집량、단백질화다당함량균유제고。결론철적불동개태대소구조적생장영향유명현차이，Fe2+교지Fe3+표현출경강적조이용효과，동양농도하，조적증식속도경쾌，영양품질경고。
Objective To study the iron with different chemical valence added through diverse mode on the nutritional and physiological properties ofChlorella pyrenoidosa.Methods Biomass and contents of chlorophyll,β-carotene, protein and carbohydrate ofChlorella pyrenoidosa were analyzed after culture with different concentration of FeSO4 and FeCl3.ResultsChlorella pyrenoidosa grew best under 12.5μmol/L FeSO4and 25μmol/L FeCl3, with the highest bio-enrichment of iron and highest contents of biomass, chlorophyll,β-carotene, protein and polysaccharide. When iron concentration increased to 100μmol/L, the growth and the physiological indices were significantly lower than those of control, and the algae medium turned yellow and even the algae died. Compared to theChlorella pyrenoidosa cultured with iron added in one portion, the contents of biomass, enrichment, protein and polysaccharide ofChlorella pyrenoidosa cultured with iron added in portions increased.Conclusion The results indicated there was significant difference in the growth ofChlorella pyrenoidosa cultured by addition of iron with different chemical valence. Fe2+ displayed stronger microalgae utilization than Fe3+. Under same concentration,Chlorella pyrenoidosa cultured by addition of Fe2+ showed the faster growth rate and higher nutritional property.
