CAJ | 학술논문

目的摸索碳纳米管-聚乙烯醇-生长因子复合敷料的制备方法,并观察其相关特性.方法通过静电纺织技术制备碳纳米管-聚乙烯醇-生长因子敷料,通过扫描电镜、细胞毒试验、人EGF ELISA试剂盒(KP98753),观察合成敷料的相关物理特性及生物学特性.结果 JSM5600-LV扫描电镜观察发现敷料显微结构稳定;24、48 h MTT细胞毒试验结果显示细胞毒性分级均为0;生长因子释放试验结果显示,12 h以前敷料的生长因子累积释放率稳步增加,无明显暴释现象,且释放的生长因子活性良好,持续释放时间长,能明显促进细胞的生长(F =334.512,P=0.000).结论本课题设计制备的碳纳米管-聚乙烯醇-生长因子复合敷料结构稳定,无细胞毒作用,能够起到缓释生长因子作用,并且释放的生长因子活性良好,能显著促进细胞的生长.
목적 모색탄납미관-취을희순-생장인자복합부료적제비방법,병관찰기상관특성.방법 통과정전방직기술제비탄납미관-취을희순-생장인자부료,통과소묘전경、세포독시험、인EGF ELISA시제합(KP98753),관찰합성부료적상관물리특성급생물학특성.결과 JSM5600-LV소묘전경관찰발현부료현미결구은정;24、48 h MTT세포독시험결과현시세포독성분급균위0;생장인자석방시험결과현시,12 h이전부료적생장인자루적석방솔은보증가,무명현폭석현상,차석방적생장인자활성량호,지속석방시간장,능명현촉진세포적생장(F =334.512,P=0.000).결론 본과제설계제비적탄납미관-취을희순-생장인자복합부료결구은정,무세포독작용,능구기도완석생장인자작용,병차석방적생장인자활성량호,능현저촉진세포적생장.
Objective To investigate the preparation method of carbon nanotube-vinol-growth factor compound dressing.Methods The carbon nanotube-vinol-growth factor compound dressing was prepared with electrostatic spinning technology.The physical properties and biological characteristics of this compound dressing were observed with scanning electron microscopy,cytotoxic test,and epidermal growth factor enzyme-linked immunosorbent assay (EGF ELISA) kit (KP98753).Results The dressing microstructure was stable with JSM5600-LV scanning electron microscopy (SEM) analysis.The 24 h and 48 h methyl thiazolyl tetrazolium (MTT) cell toxicity test showed that the cell toxicity grading was 0.The growth factor release test showed that the dressing growth factor accumulated release rate was steadily increasing before 12 h.There was not obviously critical interpretation phenomenon.The growth factor activity was good;it could obviously promote the growth of cells with long releasing time (F =334.512,P =0.000).Conclusions The carbon nanotube-vinol-growth factor compound dressing was stable,and no cytotoxic effect.It can slowly release growth factors with good activity and significantly promote cell growth.