CAJ | 학술논문

利用11对AFLP引物组合，检测杜泊羊、藏羊和小尾寒羊池DNA遗传变异，构建了各个品种的AFLP DNA指纹图谱，根据AFLP分析结果，统计了每个引物组合在各个品种中检测到的多态性条带，计算了3个品种的遗传相似系数，并据此构建了UPGMA 聚类关系图，分析了所研究羊种的遗传关系。结果表明：11对AFLP 引物组合在3个绵羊品种中共检测到312条带，平均每个引物组合产生28.36条带，品种间的遗传相似系数，其中藏羊与小尾寒羊遗传相似系数最高（0.672），杜泊羊与藏羊遗传相似系数最小（0.301），聚类结果与这些羊的育成史、分化及地理分布一致。
이용11대AFLP인물조합，검측두박양、장양화소미한양지DNA유전변이，구건료각개품충적AFLP DNA지문도보，근거AFLP분석결과，통계료매개인물조합재각개품충중검측도적다태성조대，계산료3개품충적유전상사계수，병거차구건료UPGMA 취류관계도，분석료소연구양충적유전관계。결과표명：11대AFLP 인물조합재3개면양품충중공검측도312조대，평균매개인물조합산생28.36조대，품충간적유전상사계수，기중장양여소미한양유전상사계수최고（0.672），두박양여장양유전상사계수최소（0.301），취류결과여저사양적육성사、분화급지리분포일치。
A total of 11 pairs of AFLP primer were used to detect genetic variation of pooled DNA ina sample of 3 sheep breeds DNA,and AFLP DNA fingerprinting of each sheep breed was construc -ted.Polymorphic bands and genetic similarity coefficient of 3 sheep breeds were detected and calculatedfrom AFLP data,and UPGMA cluster analysis were also performed.The 11 primer combinationsgenerated 312 polymorphic bands.The average polymorphic bands per combination was 28.36;thehighest genetic similarity coefficient of 0.672 existed between Tibetan sheep and small tail Hansheep;While the lowest similarity coefficient of 0.592 was revealed between Dorper sheep and Tibetansheep.The results were in consistent with breeding history ,differentiation and geographical distributionof those sheep breeds.