CAJ | 학술논문

采用ISSR分子标记的方法，对青海地方核桃品种单株进行了遗传关系研究。实验从30条ISSR引物中筛选出4条进行电泳分析，使用NTSYS pc 2．11F和Popgene 1．32软件进行数据统计分析并作图。结果表明：4条ISSR引物共获得40条谱带，平均每条引物10条谱带，引物平均多态带比率82．5％；4条ISSR引物可将所有样品完全分开，当遗传相似性阈值为0．736时可将样品分成7组。样品间的遗传相似性系数为0．550～0．975（平均0．763）。由于长期的自然变异和选择，青海地区核桃积累了较高的遗传多样性，单株间存在不同程度的亲缘关系。
채용ISSR분자표기적방법，대청해지방핵도품충단주진행료유전관계연구。실험종30조ISSR인물중사선출4조진행전영분석，사용NTSYS pc 2．11F화Popgene 1．32연건진행수거통계분석병작도。결과표명：4조ISSR인물공획득40조보대，평균매조인물10조보대，인물평균다태대비솔82．5％；4조ISSR인물가장소유양품완전분개，당유전상사성역치위0．736시가장양품분성7조。양품간적유전상사성계수위0．550～0．975（평균0．763）。유우장기적자연변이화선택，청해지구핵도적루료교고적유전다양성，단주간존재불동정도적친연관계。
The method of ISSR molecular markers is employed to study genetic relationship per plant of Qinghai local walnut varieties .Four ISSR primers with high polymorphism , strong signals and clear bands are selected from 30 primers.NTSYS pc 2.11F and Popgene 1.32 software are used to conduct the data analysis and plotting .The results suggest that totally 40 bands are obtained .The average number of bands is 10 per primer and the average percentage of polymorphic bands is 82. 5%.All the samples are divided into 7 groups at the similarity coefficient of 0.736.The similarity coefficient of the samples varies from 0.550 to 0.975, with an average of 0.763.Due to the long-term nature of mutation and selection , walnuts in Qinghai have accumulated high levels of genetic diversity , showed varying degrees of affinity between plants .