CAJ | 학술논문

目的：研究双泛素(diubiquitin，FAT10)表达与乳腺浸润性导管癌生物学行为的关系。方法：免疫组织化学检测50例乳腺浸润性导管癌组织中FAT10，雌激素受体(estrogen receptor，ER)、孕激素受体(progesterone receptor， PR)及人表皮生长因子受体(c-erbB2)的表达；Western印迹检测FAT10在乳腺癌细胞系MB-MDA-435和转染FAT10 siRNA表达质粒的MB-MDA-435，MCF-7和转染FAT10表达质粒的MCF-7中的表达；Transwell小室检测4种乳腺癌细胞系的侵袭能力。结果：乳腺浸润性导管癌中，FAT10的阳性表达强度与病理学分级、淋巴结转移、远处转移及TNM分期有关(P<0.01)，而与患者年龄及肿瘤大小无关(P>0.05)；受体阴性组中FAT10的阳性表达强度显著高于受体阳性组(P<0.01)，三阴乳腺癌中FAT10的阳性表达强度率显著高于受体阳性组和受体阴性组(P<0.01)；FAT10表达阳性组生存率明显低于FAT10表达阴性组(P<0.05)；Western印迹结果显示高侵袭性乳腺癌细胞系中FAT10阳性表达强度显著高于低侵袭乳腺癌细胞系；上调FAT10表达能增强乳腺癌细胞系的侵袭能力，而下调其表达能降低乳腺癌细胞系的侵袭能力(P<0.01)。结论：FAT10可能具有直接或间接增加乳腺癌细胞的侵袭能力的作用，在乳腺癌患者的侵袭和转移中起重要的作用，它有可能作为判断乳腺癌预后的又一独立指标及靶向治疗乳腺癌特别是三阴乳腺癌的潜在靶点。
목적：연구쌍범소(diubiquitin，FAT10)표체여유선침윤성도관암생물학행위적관계。방법：면역조직화학검측50례유선침윤성도관암조직중FAT10，자격소수체(estrogen receptor，ER)、잉격소수체(progesterone receptor， PR)급인표피생장인자수체(c-erbB2)적표체；Western인적검측FAT10재유선암세포계MB-MDA-435화전염FAT10 siRNA표체질립적MB-MDA-435，MCF-7화전염FAT10표체질립적MCF-7중적표체；Transwell소실검측4충유선암세포계적침습능력。결과：유선침윤성도관암중，FAT10적양성표체강도여병이학분급、림파결전이、원처전이급TNM분기유관(P<0.01)，이여환자년령급종류대소무관(P>0.05)；수체음성조중FAT10적양성표체강도현저고우수체양성조(P<0.01)，삼음유선암중FAT10적양성표체강도솔현저고우수체양성조화수체음성조(P<0.01)；FAT10표체양성조생존솔명현저우FAT10표체음성조(P<0.05)；Western인적결과현시고침습성유선암세포계중FAT10양성표체강도현저고우저침습유선암세포계；상조FAT10표체능증강유선암세포계적침습능력，이하조기표체능강저유선암세포계적침습능력(P<0.01)。결론：FAT10가능구유직접혹간접증가유선암세포적침습능력적작용，재유선암환자적침습화전이중기중요적작용，타유가능작위판단유선암예후적우일독립지표급파향치료유선암특별시삼음유선암적잠재파점。
Objective: To study the relationship between FAT10 expression and biological behaviors in inif trating ductal carcinoma of breast. Methods: The expressions of diubiquitin (FAT10), estrogen receptor (ER), progesterone receptor (PR) and c-erbB2 in 50 cases of inif trating ductal carcinoma of breast were detected by immunohistochemistry. Western blot was used to detect FAT10 expression in MB-MDA-435, MB-MDA-435-transfected with FAT10 siRNA expression plasmid, MCF-7 and MCF-7-transfected with FAT10 expression plasmid, respectively. Transwell was used to detect invasion capability of MB-MDA-435, MB-MDA-435-transfected with FAT10 siRNA expression plasmid, MCF-7 and MCF-7-transfected with FAT10 expression plasmid. Results: hTe expression intensity of FAT10 was signiifcantly correlated to patho-grading, lymph nodes metastasis, distant metastasis and TNM staging (P<0.01), but not to age of patients and tumor sizes in iniftrating ductal carcinoma of breast (P>0.05). hTe expression intensity of FAT10 in receptor- negative group was obviously stronger than that in receptor- positive group (P<0.01). hTe expression intensity of FAT10 in triple-negative breast cancer was signiifcantly stronger than that in non- triple-negative breast cancer (P<0.01). hTe survival rate of patients with FAT10 positive expression was significantly lower than negative ones (P<0.05). Western blot results showed that FAT10 intensity in MB-MDA-435 significantly higher than that in MCF-7. Up-regulation expression of FAT10 could obviously increase the invasion capability of MCF-7, and down-regulation of FAT10 could signiifcantly decrease the invasion capability of MB-MDA-435 (P<0.01). Conclusion: FAT10 might increase the invasion capability of breast cancer cells by direct or indirect ways, and play an important role in invasion and metastasis of breast cancer. FAT10 might be an independent index for evaluation of breast cancer prognosis, and a potential target for breast cancer therapy, especially for triple-negative breast cancer.