中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2014年
12期
1259-1265
,共7页
白藜芦醇%失血性休克%肠损伤%氧化应激%线粒体
白藜蘆醇%失血性休剋%腸損傷%氧化應激%線粒體
백려호순%실혈성휴극%장손상%양화응격%선립체
resveratrol%hemorrhagic shock%intestinal injury%oxidative stress%mitochondria
目的：探讨白藜芦醇是否能够减轻失血性休克大鼠肠损伤及其作用机制。方法：24只无特定病原体级Sprague-Dawley大鼠随机分为正常对照组(n=8)、白藜芦醇治疗组(SR组，n=8)和溶剂组(SS组，n=8)，记录平均动脉压。失血性休克2 h后分别给予15 mg/kg的白藜芦醇或0.3 mL等体积的溶剂(70%乙醇)并回输自体血。治疗2 h后采集小肠标本行HE染色后，再行肠黏膜的病理学观察和评分，免疫组织化学法检测超氧化物歧化酶2(superoxide dismutase 2，SOD2)表达，Western印迹测定SOD2和细胞色素C(cytochrome C，Cyt C)蛋白含量。取部分肠组织匀浆检查ATP、谷胱甘肽过氧化物酶(glutathione peroxidase，GXH-px)、过氧化氢酶(catalase，CAT)、总SOD活性。结果：自体血回输2 h后，SR组平均动脉血压高于SS组，两组间差异有统计学意义(P<0.01)；SR组较SS组病理损伤减轻，且病理学评分明显下降(P<0.05)；SR组小肠组织匀浆GXH-px，CAT和SOD活性以及ATP水平均高于SS组(均P<0.01)；SR组SOD2蛋白水平高于SS组，胞质Cyt C水平明显低于SS组，组间比较差异有统计学意义(均P<0.01)。结论：白藜芦醇减轻了失血性休克大鼠的肠损伤，其机制可能与减少氧化应激和保护线粒体有关。
目的：探討白藜蘆醇是否能夠減輕失血性休剋大鼠腸損傷及其作用機製。方法：24隻無特定病原體級Sprague-Dawley大鼠隨機分為正常對照組(n=8)、白藜蘆醇治療組(SR組，n=8)和溶劑組(SS組，n=8)，記錄平均動脈壓。失血性休剋2 h後分彆給予15 mg/kg的白藜蘆醇或0.3 mL等體積的溶劑(70%乙醇)併迴輸自體血。治療2 h後採集小腸標本行HE染色後，再行腸黏膜的病理學觀察和評分，免疫組織化學法檢測超氧化物歧化酶2(superoxide dismutase 2，SOD2)錶達，Western印跡測定SOD2和細胞色素C(cytochrome C，Cyt C)蛋白含量。取部分腸組織勻漿檢查ATP、穀胱甘肽過氧化物酶(glutathione peroxidase，GXH-px)、過氧化氫酶(catalase，CAT)、總SOD活性。結果：自體血迴輸2 h後，SR組平均動脈血壓高于SS組，兩組間差異有統計學意義(P<0.01)；SR組較SS組病理損傷減輕，且病理學評分明顯下降(P<0.05)；SR組小腸組織勻漿GXH-px，CAT和SOD活性以及ATP水平均高于SS組(均P<0.01)；SR組SOD2蛋白水平高于SS組，胞質Cyt C水平明顯低于SS組，組間比較差異有統計學意義(均P<0.01)。結論：白藜蘆醇減輕瞭失血性休剋大鼠的腸損傷，其機製可能與減少氧化應激和保護線粒體有關。
목적：탐토백려호순시부능구감경실혈성휴극대서장손상급기작용궤제。방법：24지무특정병원체급Sprague-Dawley대서수궤분위정상대조조(n=8)、백려호순치료조(SR조，n=8)화용제조(SS조，n=8)，기록평균동맥압。실혈성휴극2 h후분별급여15 mg/kg적백려호순혹0.3 mL등체적적용제(70%을순)병회수자체혈。치료2 h후채집소장표본행HE염색후，재행장점막적병이학관찰화평분，면역조직화학법검측초양화물기화매2(superoxide dismutase 2，SOD2)표체，Western인적측정SOD2화세포색소C(cytochrome C，Cyt C)단백함량。취부분장조직균장검사ATP、곡광감태과양화물매(glutathione peroxidase，GXH-px)、과양화경매(catalase，CAT)、총SOD활성。결과：자체혈회수2 h후，SR조평균동맥혈압고우SS조，량조간차이유통계학의의(P<0.01)；SR조교SS조병리손상감경，차병이학평분명현하강(P<0.05)；SR조소장조직균장GXH-px，CAT화SOD활성이급ATP수평균고우SS조(균P<0.01)；SR조SOD2단백수평고우SS조，포질Cyt C수평명현저우SS조，조간비교차이유통계학의의(균P<0.01)。결론：백려호순감경료실혈성휴극대서적장손상，기궤제가능여감소양화응격화보호선립체유관。
Objective: To explore whether resveratrol can reduce intestinal damage in hemorrhagic shock rats and the underlying mechanism. Methods: A total of 24 Sprague-Dawley rats of speciif c pathogen free (SPF) were randomly divided into a control group(n=8), a resveratrol group (SR group,n=8) and a vehicle group (SS group,n=8). hT e mean arterial pressure was recorded. Two hours atf er hemorrhagic shock, 15 mg/kg resveratrol or 0.3 mL equal volume of vehicle and autologous blood were given, respectively. The intestinalspecimens were collected for hematoxylin-eosin (HE) staining and calculated the pathological score. The superoxide dismutase 2 (SOD2) and cytochrome C (Cyt C) protein expression was examined by immunohistochemistry and/or Western blot. ATP level, activities of glutathione peroxidase (GXH-px), catalase (CAT) and SOD were also detected. Results: Two hours atfer autologous blood transfusion, the mean arterial pressure in the SR group was signiifcantly higher than that in the SS group (P<0.01). Compared with the SS group, the pathological injury was signiifcantly alleviated and pathological scores were dramatically reduced in the SR group (P<0.05). hTe activities of GXH-px, CAT, SOD and the ATP levels in the SR group were signiifcantly higher than those in the SS group (allP<0.01). Compared with the SS group, the SOD2 expression was significantly higher while the Cyt C expression was dramatically lower in the SR group (both P<0.01). Conclusion: Resveratrol could alleviate the intestinal injury in hemorrhagic shock rats, which might be associated with its effects on reduction of oxidative stress and protection of mitochondria.
