中南大学学报（医学版）
中南大學學報（醫學版）
중남대학학보（의학판）
JOURNAL OF CENTRAL SOUTH UNIVERSITY (MEDICAL SCIENCES)
2014年
12期
1228-1232
,共5页
肿瘤坏死因子转换酶%黏蛋白5AC%人中性粒细胞弹性蛋白酶%肿瘤坏死因子转换酶抑制剂-1
腫瘤壞死因子轉換酶%黏蛋白5AC%人中性粒細胞彈性蛋白酶%腫瘤壞死因子轉換酶抑製劑-1
종류배사인자전환매%점단백5AC%인중성립세포탄성단백매%종류배사인자전환매억제제-1
TNF-α converting enzyme%MUC5AC%human neutrophil elastae%TNF-α converting enzyme inhibitor-1
目的：探讨肿瘤坏死因子转换酶(TNF-α converting enzyme，TACE)对炎症条件下气道上皮细胞形成黏液高分泌过程的影响。方法：通过不同剂量人中性粒细胞弹性蛋白酶(human neutrophil elastase，HNE)刺激人肺腺癌的气道上皮细胞A549，构建炎症条件下气道黏液高分泌模型，以TACE抑制剂-1(TNF-α converting enzyme inhibitor-1，TAPI-1)进行干预，观察TACE、黏蛋白(mucin，MUC)5AC的表达。将A549细胞分为5组：对照组，HNE(15，25，50 nmol/L)组，TAPI-1组。RT-PCR检测各组TACE和MUC5AC mRNA的表达；Western印迹检测TACE蛋白的表达；酶联免疫吸附测定法(ELISA)观察MUC5AC蛋白的表达。结果：各剂量HNE处理后，TACE和MUC5AC的mRNA和蛋白表达均较对照组明显升高(P<0.01)，且呈现剂量依赖性。而给予TAPI-1预处理后，与HNE刺激组比，各指标明显下调(P<0.01)。结论：TACE参与了黏液高分泌的细胞转导途径，并可促成炎性气道黏液高分泌的形成。
目的：探討腫瘤壞死因子轉換酶(TNF-α converting enzyme，TACE)對炎癥條件下氣道上皮細胞形成黏液高分泌過程的影響。方法：通過不同劑量人中性粒細胞彈性蛋白酶(human neutrophil elastase，HNE)刺激人肺腺癌的氣道上皮細胞A549，構建炎癥條件下氣道黏液高分泌模型，以TACE抑製劑-1(TNF-α converting enzyme inhibitor-1，TAPI-1)進行榦預，觀察TACE、黏蛋白(mucin，MUC)5AC的錶達。將A549細胞分為5組：對照組，HNE(15，25，50 nmol/L)組，TAPI-1組。RT-PCR檢測各組TACE和MUC5AC mRNA的錶達；Western印跡檢測TACE蛋白的錶達；酶聯免疫吸附測定法(ELISA)觀察MUC5AC蛋白的錶達。結果：各劑量HNE處理後，TACE和MUC5AC的mRNA和蛋白錶達均較對照組明顯升高(P<0.01)，且呈現劑量依賴性。而給予TAPI-1預處理後，與HNE刺激組比，各指標明顯下調(P<0.01)。結論：TACE參與瞭黏液高分泌的細胞轉導途徑，併可促成炎性氣道黏液高分泌的形成。
목적：탐토종류배사인자전환매(TNF-α converting enzyme，TACE)대염증조건하기도상피세포형성점액고분비과정적영향。방법：통과불동제량인중성립세포탄성단백매(human neutrophil elastase，HNE)자격인폐선암적기도상피세포A549，구건염증조건하기도점액고분비모형，이TACE억제제-1(TNF-α converting enzyme inhibitor-1，TAPI-1)진행간예，관찰TACE、점단백(mucin，MUC)5AC적표체。장A549세포분위5조：대조조，HNE(15，25，50 nmol/L)조，TAPI-1조。RT-PCR검측각조TACE화MUC5AC mRNA적표체；Western인적검측TACE단백적표체；매련면역흡부측정법(ELISA)관찰MUC5AC단백적표체。결과：각제량HNE처리후，TACE화MUC5AC적mRNA화단백표체균교대조조명현승고(P<0.01)，차정현제량의뢰성。이급여TAPI-1예처리후，여HNE자격조비，각지표명현하조(P<0.01)。결론：TACE삼여료점액고분비적세포전도도경，병가촉성염성기도점액고분비적형성。
Objective: To investigate the effect of tumor necrosis factor-α converting enzyme (TACE) on mucous hypersecretion in inlf ammatory airway. Methods: Mucous hypersecretion model of human lung adenocarcinoma cells A549 was induced by human neutrophil elastase (HNE), and TNF-α converting enzyme inhibitor-1 (TAPI-1), an inhibitor of TACE, was chosen for the inference study. The expression of MUC5AC and TACE was examined. hT e cells were divided into 5 groups: a negative control group, HNE1 (15 nmol/L) group, HNE2 (25 nmol/L) group, HNE3 (50 nmol/L) group and TAPI-1 group. RT-PCR was used to examine MUC5AC and TACE mRNA expression. The protein expression of TACE and MUC5AC was examined by Western blot and ELISA, respectively. Results: HNE induced the TACE and MUC5AC mRNA and protein expression in a dose-dependent manner. Compared with the control group, the increases were all signiifcantly increased in the three dosages of HNE group (P<0.01). The HNE-induced TACE and MUC5AC mRNA and protein expression were dramatically attenuated in the presence of TAPI-1, an inhibitor of TACE (P<0.01). Conclusion: TACE participated cell signalling pathway of airway mucous hypersecretion, and could down regulation the level of inlfammation airway mucous hypersecretion.
