CAJ | 학술논문

利用快速筛选方法从大庆采油三厂油田采出水中筛选出高产生物表面活性剂菌株 S2,经鉴定为芽孢杆菌属.对 S2菌株产生的生物表面活性剂进行提取及纯化后,通过离子鉴别实验及红外光谱法对其进行定性检测,确定其种类为非离子型脂肽类.以菌种 S2产生的发酵液的乳化指数(E 24)为指标,通过响应面优化方法对 S2菌株的培养条件进行优化,以接种量、pH 值、温度、摇床转速为因素优化对象进行实验.在此模型的基础上,通过二次回归方程得出最佳值是 pH 为7.2、温度为43.5℃、接种量为5.2%(V/V )、摇床转速为162 r/min.在优化后的最佳培养条件下,测得菌株 S2所产生发酵液的最优 E 24为81.20%.
이용쾌속사선방법종대경채유삼엄유전채출수중사선출고산생물표면활성제균주 S2,경감정위아포간균속.대 S2균주산생적생물표면활성제진행제취급순화후,통과리자감별실험급홍외광보법대기진행정성검측,학정기충류위비리자형지태류.이균충 S2산생적발효액적유화지수(E 24)위지표,통과향응면우화방법대 S2균주적배양조건진행우화,이접충량、pH 치、온도、요상전속위인소우화대상진행실험.재차모형적기출상,통과이차회귀방정득출최가치시 pH 위7.2、온도위43.5℃、접충량위5.2%(V/V )、요상전속위162 r/min.재우화후적최가배양조건하,측득균주 S2소산생발효액적최우 E 24위81.20%.
S2 strain that highly produces biosurfactant was screened from the oilfield water of three plants in Daqing Oilfield in the rapid screening method,and the S2 strain was identified as Bacillus sp..Through FT-IR and ion identification test,S2 biosurfactant was identified as nonionic lipopeptide biosurfactant.This experiment treated emul-sification index (E24 )of S2 strain fermentation broth as the object.Through response surface optimization method, the culture conditions (inoculum,pH,temperature and rotation speed)of the S2 strain were optimized.On the basis of response surface model,the optimum pH value was 7.2 and the temperature was 43.5 ℃,inoculation of 5.2% (V/V),rotation speed was 162 r/min through the quadratic regression equation.Under the optimal condition,the best E24 of S2 strain fermentation broth was 81.20%.