CAJ | 학술논문

采用高效液相色谱质谱法分析了湘江长沙河段水体及底泥中4种常见四环素类抗生素的含量特征及其季节变化,并采用实时荧光定量 PCR 研究了该水域相应的5种代表性抗生素抗性基因(tet A,tet C,tet G,tet M 和 tet X)的存在及丰度.结果表明,水体中四环素类抗生素的质量浓度均值在16.23~496.73 ng·L-1之间,底泥中四环素类抗生素的质量分数为347.77~3829.75 ng·kg-1,易受季节变化影响.5种目标抗性基因在所有样品中均有检出,其中 tet A 和 tet C 的绝对拷贝数及相对丰度较高,为湘江长沙河段的优势抗性基因.相关性分析表明：冬季水体中的 tet A 和 tet C 含量与底泥中的含量显著相关,几种目标抗生素与抗性基因之间并不存在明显线性关系.
채용고효액상색보질보법분석료상강장사하단수체급저니중4충상견사배소류항생소적함량특정급기계절변화,병채용실시형광정량 PCR 연구료해수역상응적5충대표성항생소항성기인(tet A,tet C,tet G,tet M 화 tet X)적존재급봉도.결과표명,수체중사배소류항생소적질량농도균치재16.23~496.73 ng·L-1지간,저니중사배소류항생소적질량분수위347.77~3829.75 ng·kg-1,역수계절변화영향.5충목표항성기인재소유양품중균유검출,기중 tet A 화 tet C 적절대고패수급상대봉도교고,위상강장사하단적우세항성기인.상관성분석표명：동계수체중적 tet A 화 tet C 함량여저니중적함량현저상관,궤충목표항생소여항성기인지간병불존재명현선성관계.
This paper adopted high-performance liquid chromatography-mass spectrograph (HPLC-MS)to investigate the occurrence and seasonal changes of tetracycline antibiotics in the Xiang River and its sediments.Five representative antibiotic resistance genes (tet A,tet C,tet G,tet M,tet X)in water and sediment samples were quantified with real-time PCR.The concentrations of tetracycline showed sensitive seasonal changes,ranging from 1 6.23 ng·L-1 to 496.73 ng·L-1 in the water samples,and from 347.77 ng·kg-1 to 3 829.75 n·kg-1 in the sediment samples,respectively.The target resistance genes were de-tected in all samples.Compared with other resistance genes,tet A and tet C were higher in absolute copy number and relative abundance,suggesting that the tet A and tet C were dominant in the Xiang River.Sta-tistical analysis indicated a positive correlation between the relative abundance of tet A and tet C in the wa-ter samples and the sediment samples in winter.There was no linearly relationship between antibiotics and resistance genes.