CAJ | 학술논문

目的：探讨兔腰椎结核动物模型的 CT、MRI 表现，为诊断腰椎结核提供影像学依据。方法健康新西兰白兔60只，通过腹部侧方入路手术方式将人型结核杆菌 H37Rv 标准菌株进行新西兰白兔腰椎种植，构建椎体结核动物模型，术后4、8 w 时行 CT 及 MRI 检查，并对病变行组织病理学检查。结果成功建立新西兰白兔腰椎结核动物模型。应用 MRI 常规检查方法及 DWI 检查可以早期发现椎体是否感染结核；且在术后第4、8周时MRI 诊断脊柱结核不同病变的敏感性高于 CT；病理学 HE 染色检查显示实验组病变椎体、椎旁软组织内可见脓细胞、类上皮样细胞及坏死组织。结论在新西兰白兔腰椎椎体进行结核标准菌株的种植感染，通过病理学检查证实可以构建出脊柱结核动物模型；CT、MRI 能够为疾病早期诊断提供影像学依据，磁共振成像中弥散加权成像（DWI）序列更为敏感。
목적：탐토토요추결핵동물모형적 CT、MRI 표현，위진단요추결핵제공영상학의거。방법건강신서란백토60지，통과복부측방입로수술방식장인형결핵간균 H37Rv 표준균주진행신서란백토요추충식，구건추체결핵동물모형，술후4、8 w 시행 CT 급 MRI 검사，병대병변행조직병이학검사。결과성공건립신서란백토요추결핵동물모형。응용 MRI 상규검사방법급 DWI 검사가이조기발현추체시부감염결핵；차재술후제4、8주시MRI 진단척주결핵불동병변적민감성고우 CT；병이학 HE 염색검사현시실험조병변추체、추방연조직내가견농세포、류상피양세포급배사조직。결론재신서란백토요추추체진행결핵표준균주적충식감염，통과병이학검사증실가이구건출척주결핵동물모형；CT、MRI 능구위질병조기진단제공영상학의거，자공진성상중미산가권성상（DWI）서렬경위민감。
Objective To provide an animal model for basic research of bone tuberculosis.Methods Sixty healthy New Zealand rabbits were selected in this study.Firstly,animal models of spinal tuberculosis were made by operation,then they were examined with imaging and pathology methods at 4 weeks and 8 weeks respectively.Results The spinal tuberculosis model was successfully established in rabbits.Application of conventional MRI method and DWI method can be detected at an early stage bone which is infected with tuberculosis;and after 4 week,8 weeks,the sensitivity of MRI diagnosing spine tuberculosis is higher than that of CT.For the group with infections,pus,epithelioid cells or necrotic foci formation could be seen from vertebrae,paraspinal soft tissue sections according to the HE staining.Conclusion Rabbit spinal tuberculosis disease model can be successfully established though planting standard strains of human type Mycobacterium tuberculosis H37Rv on healthy rabbits lumbar.CT,MRI can provide early diagnosis of spinal tuberculosis with some information,DWI in MRI scanning method is more sensitive to early diag-nosis of spinal tuberculosis.