CAJ | 학술논문

为探讨 microRNAs （miRNAs）在小麦镉胁迫不同时间段的差异表达规律，初步鉴定参与小麦镉胁迫响应的 miRNAs。以郑麦4号为材料，采用实时定量 PCR 技术研究小麦幼苗在不同时间镉胁迫（0 h，6 h，12 h，24 h，48 h）后9个 miRNAs 的差异表达规律。结果表明：miRNA319和 miRNA397在叶片和根中的表达呈不同程度的上调，而 miRNA399和 miRNA167在叶片和根中的表达呈不同程度的下调，暗示小麦幼苗中9个 miRNAs 参与了镉胁迫响应的调控。进一步对9个 miRNAs 作用的靶基因进行预测和分析发现，它们主要参与植物逆境条件下的信号传导、生长发育和胁迫应答等过程，可能在调控小麦对镉胁迫的反应中起着重要作用。
위탐토 microRNAs （miRNAs）재소맥력협박불동시간단적차이표체규률，초보감정삼여소맥력협박향응적 miRNAs。이정맥4호위재료，채용실시정량 PCR 기술연구소맥유묘재불동시간력협박（0 h，6 h，12 h，24 h，48 h）후9개 miRNAs 적차이표체규률。결과표명：miRNA319화 miRNA397재협편화근중적표체정불동정도적상조，이 miRNA399화 miRNA167재협편화근중적표체정불동정도적하조，암시소맥유묘중9개 miRNAs 삼여료력협박향응적조공。진일보대9개 miRNAs 작용적파기인진행예측화분석발현，타문주요삼여식물역경조건하적신호전도、생장발육화협박응답등과정，가능재조공소맥대력협박적반응중기착중요작용。
In order to delve into the differential expression of microRNAs (miRNAs)in wheat treated with cadmium stress for different time,in this study,wheat seedlings of Zhengmai 4 were treated with different time (0,6 h,12 h,24 h,48 h)under cadmium stress.Real-time PCR was used to detect the differential expression of nine miRNAs under cadmium stress.Results:Expression of miRNA319 and miRNA397 increased in varying degrees in leaves and roots,while miRNA399 and miRNA167 decreased, indicating that the nine miRNAs participated in regulation of cadmium stress response.The analysis on the target genes of these miRNAs revealed that these miRNAs participated in the signal transduction,growth and development and stress response under abiotic stress and might play important roles in wheat under cadmium stress.