CAJ | 학술논문

目的：研究和探讨嗅鞘细胞静脉移植修复脊髓损伤的疗效与移植剂量的关系，探求较佳的静脉移植剂量。方法制备Wistar大鼠T10脊髓左侧半切模型，随机分成五组：嗅鞘细胞大剂量移植组（ A组）、嗅鞘细胞中剂量移植组（B组）、嗅鞘细胞小剂量移植组（C组）、培养液D/F12移植组（D组）、空白对照组（E组）。定期行大鼠神经功能评价及组织形态学观察，评价脊髓恢复情况。结果 A、B组神经功能恢复和组织学改变与C、D、E组有显著差别（P<0.05）；C组与D、E组间虽有差别（P<0.05），但是其差别较小；A、B组之间无显著差别（P>0.05）；D、E两组无显著差别（P>0.05）。结论嗅鞘细胞静脉移植对修复脊髓损伤有一定的疗效，其疗效与移植剂量在一定范围内存在正向关系。细胞移植的较佳剂量为2×106，低于此剂量则不能达到良好的疗效，多于此剂量，疗效无明显提高。此种移植方式既使操作简单容易，又具有较好的疗效，并且可以重复多次进行，具有良好的临床推广和应用价值。
목적：연구화탐토후초세포정맥이식수복척수손상적료효여이식제량적관계，탐구교가적정맥이식제량。방법제비Wistar대서T10척수좌측반절모형，수궤분성오조：후초세포대제량이식조（ A조）、후초세포중제량이식조（B조）、후초세포소제량이식조（C조）、배양액D/F12이식조（D조）、공백대조조（E조）。정기행대서신경공능평개급조직형태학관찰，평개척수회복정황。결과 A、B조신경공능회복화조직학개변여C、D、E조유현저차별（P<0.05）；C조여D、E조간수유차별（P<0.05），단시기차별교소；A、B조지간무현저차별（P>0.05）；D、E량조무현저차별（P>0.05）。결론후초세포정맥이식대수복척수손상유일정적료효，기료효여이식제량재일정범위내존재정향관계。세포이식적교가제량위2×106，저우차제량칙불능체도량호적료효，다우차제량，료효무명현제고。차충이식방식기사조작간단용역，우구유교호적료효，병차가이중복다차진행，구유량호적림상추엄화응용개치。
Objective:To compare the efficacy of different doses of olfactory ensheathing cells vein transplantation for treat-ment of acute spinal cord injury,and the correlation between dose and efficacy. Methods:The Wistar rat model was prepared by the left T10 spinal cord hemisection. The rats were randomly divided into five groups:high-dose of olfactory ensheathing cells vein transplantation group( group A),intermediate-dose of olfactory ensheathing cells vein transplantation group( group B),low-dose of olfactory ensheathing cells vein transplantation group( group C),the culture medium D/F12 vein transplan-tation group( group D),the blanK control group( group E). The spinal cord restoration was evaluated by the neurological function with a modified CBS score system and histological examination. Results:There was significant difference between group A/B and group C/D/E for the neurological recovery and histological changes(P<0. 05);There was difference between C and D/E,but it was little. There was no significant difference between group D and group E( P>0. 05);with no differ-ence between group A and group B(P>0. 05). Conclusion:The transplantation of olfactory ensheathing cells by vein has a certain effect on treating SCI,and there is a certain positive relationship between the efficacy and transplant dose. The appro-priate dose is 2 × 106 ,a less dose can not achieve a good effect,and there is no significant improvement with higher dose. This method is simply and easily-operated and it has a good perspective for clinical practice and application.