临床眼科杂志
臨床眼科雜誌
림상안과잡지
JOURNAL OF CLINICAL OPHTHALMOLOGY
2015年
3期
267-270
,共4页
姜黄素%新生血管%角膜碱烧伤%NADPH氧化酶4
薑黃素%新生血管%角膜堿燒傷%NADPH氧化酶4
강황소%신생혈관%각막감소상%NADPH양화매4
Curcumin%Corneal neovascularization (CNV)%Corneal alkaline burn%NOX4
目的研究姜黄素对大鼠角膜碱烧伤新生血管的抑制作用和对角膜NADPH氧化酶4（NADPH oxi-dase 4）表达的影响。方法选用标准化清洁级雄性SD大鼠24只，随机分为4组，每组6只，右眼为实验眼。A组为空白对照组，不做任何处理，B组为碱烧伤模型组，C组为姜黄素治疗组，D组为二甲基亚砜（DMSO）对照组。B、C和D组分别以直径3 mm的圆形滤纸片浸入1 mol／ L NaOH溶液中20 s，均匀的贴于大鼠右眼角膜中央40 s，制造大鼠角膜碱烧伤模型，B组结膜下注射生理盐水0．1 ml，C组注射姜黄素溶液0．1 ml，D组注射姜黄素溶剂DMSO 0．1 ml，均隔天注射1次，注射7次。第3天、第7天、第10天、第14天裂隙灯下观察并照相记录角膜新生血管（ CNV）生长情况，计算CNV面积，数据做统计学分析。第14天处死所有大鼠，摘取右眼球进行石蜡包埋切片，H-E染色观察角膜形态学变化，免疫组化法（S-P）观察角膜NOX4表达，计算机彩色图像分析软件计算平均光密度值，数据做统计学分析。结果 C组新生血管第14天时明显减少退化，角膜透亮，CNV 面积和B 组比较有统计学差异；H-E染色C组角膜基质层新生血管减少退化；免疫组化A组NOX4仅在角膜上皮层和内皮层微量表达，B组较A组明显表达，C组和B组比较表达下降有统计学差异，D组和B组比较无统计学差异。结论姜黄素可以有效的抑制大鼠角膜碱烧伤新生血管，其效应与抗氧化抑制NOX4表达有关。
目的研究薑黃素對大鼠角膜堿燒傷新生血管的抑製作用和對角膜NADPH氧化酶4（NADPH oxi-dase 4）錶達的影響。方法選用標準化清潔級雄性SD大鼠24隻，隨機分為4組，每組6隻，右眼為實驗眼。A組為空白對照組，不做任何處理，B組為堿燒傷模型組，C組為薑黃素治療組，D組為二甲基亞砜（DMSO）對照組。B、C和D組分彆以直徑3 mm的圓形濾紙片浸入1 mol／ L NaOH溶液中20 s，均勻的貼于大鼠右眼角膜中央40 s，製造大鼠角膜堿燒傷模型，B組結膜下註射生理鹽水0．1 ml，C組註射薑黃素溶液0．1 ml，D組註射薑黃素溶劑DMSO 0．1 ml，均隔天註射1次，註射7次。第3天、第7天、第10天、第14天裂隙燈下觀察併照相記錄角膜新生血管（ CNV）生長情況，計算CNV麵積，數據做統計學分析。第14天處死所有大鼠，摘取右眼毬進行石蠟包埋切片，H-E染色觀察角膜形態學變化，免疫組化法（S-P）觀察角膜NOX4錶達，計算機綵色圖像分析軟件計算平均光密度值，數據做統計學分析。結果 C組新生血管第14天時明顯減少退化，角膜透亮，CNV 麵積和B 組比較有統計學差異；H-E染色C組角膜基質層新生血管減少退化；免疫組化A組NOX4僅在角膜上皮層和內皮層微量錶達，B組較A組明顯錶達，C組和B組比較錶達下降有統計學差異，D組和B組比較無統計學差異。結論薑黃素可以有效的抑製大鼠角膜堿燒傷新生血管，其效應與抗氧化抑製NOX4錶達有關。
목적연구강황소대대서각막감소상신생혈관적억제작용화대각막NADPH양화매4（NADPH oxi-dase 4）표체적영향。방법선용표준화청길급웅성SD대서24지，수궤분위4조，매조6지，우안위실험안。A조위공백대조조，불주임하처리，B조위감소상모형조，C조위강황소치료조，D조위이갑기아풍（DMSO）대조조。B、C화D조분별이직경3 mm적원형려지편침입1 mol／ L NaOH용액중20 s，균균적첩우대서우안각막중앙40 s，제조대서각막감소상모형，B조결막하주사생리염수0．1 ml，C조주사강황소용액0．1 ml，D조주사강황소용제DMSO 0．1 ml，균격천주사1차，주사7차。제3천、제7천、제10천、제14천렬극등하관찰병조상기록각막신생혈관（ CNV）생장정황，계산CNV면적，수거주통계학분석。제14천처사소유대서，적취우안구진행석사포매절편，H-E염색관찰각막형태학변화，면역조화법（S-P）관찰각막NOX4표체，계산궤채색도상분석연건계산평균광밀도치，수거주통계학분석。결과 C조신생혈관제14천시명현감소퇴화，각막투량，CNV 면적화B 조비교유통계학차이；H-E염색C조각막기질층신생혈관감소퇴화；면역조화A조NOX4부재각막상피층화내피층미량표체，B조교A조명현표체，C조화B조비교표체하강유통계학차이，D조화B조비교무통계학차이。결론강황소가이유효적억제대서각막감소상신생혈관，기효응여항양화억제NOX4표체유관。
Objective To study the inhibition of curcumin on corneal neovascularization (CNV)in the corneal al-kaline-burned-rat(ABR)and the effect of curcumin on expression in corneal NADPH oxidase 4. Methods 24 male SD rats were randomly divided into 4 groups with 6 per group and the right eye was observed. A group was blank control group, B group was alkaline-burned negative control group,C group was curcumin treatment group and D group was solvent control group. The rat alkaline burn model was made respectively by pasting the their right eyes for 40 s with a 3 mm round filter paper sheet which has been immersed into 1 mol/ L NaOH solution for 20 s. B groups were subconjunctival injected 0. 9%saline 0. 1ml every other day,C groups were injected 40 uM curcumin 0. 1ml,D groups were injected 5mg/ ml dexametha-sone 0. 1ml and total injections were 7 times. CNV was observed on the 3rd day,7th day,10th day and 14th day under slit-lamp microscope and calculated CNV area. All rats were sacrificed and took full eyes paraffin embedding on the 14th day. The pathological change was observed by H-E staining and the expression of NOX4 in corneal was detected by immunohisto-chemical method. Results CNV of C group decreased obviously on the 14th day. According to the comparison between B group and C group,CNV area was statistically significant. CNV of corneal stromal layer degenerated in H-E staining. In A group,NOX4 was low expression in epithelium and endothelium of cornea. NOX4 of B group was expressed obviously and C group was lower than B group,which had a statistically significant. In addition,there was no significant difference be-tween D group and B group. Conclusion Curcumin is capable of inhibiting CNV effectively in the corneal ABR,and its effect is determined by antioxidant inhibiting the cornea NOX4 expression.
