CAJ | 학술논문

目的比较硫酸阿托品滴眼液滴眼和氢溴酸东莨菪碱皮下注射制作水液缺乏型干眼兔模型各自的特点。方法健康新西兰大白兔18只，随机分为正常组、阿托品组、东莨菪碱组3组。正常组6只兔不接受任何处理；阿托品组6只兔双眼滴1％硫酸阿托品滴眼液；东莨菪碱组6只兔皮下注射氢溴酸东莨菪碱注射剂。3组分别于实验前及实验第3、7、21、28天检查Schirmer I试验、泪膜破裂时间、角膜荧光素染色。结果东莨菪碱组在实验第7、14、21、28天，Schirmer I试验、泪膜破裂时间和角膜荧光染色3项检查与实验前相比（P ＜0．05），具有明显差异；阿托品组在实验第3、7、14天，3项检查与实验前相比（P ＜0．05），差异具有意义，且第7天差异达到最大，至实验第21天时，P ＞0．05，无明显差异；实验第3天东莨菪碱组比阿托品组相比（P ＜0．05），差异具有显著意义；实验第21天东莨菪碱组比阿托品组（P ＜0．05），差异具有义。泪腺病理学检查东莨菪碱组较阿托品组明显有大片炎细胞浸润，腺上皮细胞萎缩。结论硫酸阿托品滴眼液点眼制作的干眼模型经济、便捷、起效快，但不能持久；氢溴酸东莨菪碱注射剂皮下注射制作干眼模型起效较慢，但稳定长久，并对泪腺上皮细胞影响明显。
목적비교류산아탁품적안액적안화경추산동랑탕감피하주사제작수액결핍형간안토모형각자적특점。방법건강신서란대백토18지，수궤분위정상조、아탁품조、동랑탕감조3조。정상조6지토불접수임하처리；아탁품조6지토쌍안적1％류산아탁품적안액；동랑탕감조6지토피하주사경추산동랑탕감주사제。3조분별우실험전급실험제3、7、21、28천검사Schirmer I시험、루막파렬시간、각막형광소염색。결과동랑탕감조재실험제7、14、21、28천，Schirmer I시험、루막파렬시간화각막형광염색3항검사여실험전상비（P ＜0．05），구유명현차이；아탁품조재실험제3、7、14천，3항검사여실험전상비（P ＜0．05），차이구유의의，차제7천차이체도최대，지실험제21천시，P ＞0．05，무명현차이；실험제3천동랑탕감조비아탁품조상비（P ＜0．05），차이구유현저의의；실험제21천동랑탕감조비아탁품조（P ＜0．05），차이구유의。루선병이학검사동랑탕감조교아탁품조명현유대편염세포침윤，선상피세포위축。결론류산아탁품적안액점안제작적간안모형경제、편첩、기효쾌，단불능지구；경추산동랑탕감주사제피하주사제작간안모형기효교만，단은정장구，병대루선상피세포영향명현。
Objective To compare atropine sulfate eye drops and subcutaneous scopolamine hydrobromide pro-duced aqueous-deficient dry eye rabbit model of their own characteristics. Methods 18 healthy New Zealand white rab-bits were randomly divided into normal group,the atropine group,scopolamine group three groups. Normal group six rab-bits do not accept any treatment;atropine group six rabbits eye drops of 1% atropine sulfate eye drops;scopolamine group six rabbits subcutaneous injections of scopolamine hydrobromide. 3 groups were examined before the experiment and the ex-periment on day 3,7,21,28 Schirmer I test,tear film break-up time,corneal fluorescein staining. Results Scopolamine 7,14,21 and 28 days in the experimental group,Schirmer I test,tear film break-up time and corneal staining compared with the experimental examination 3 (P < 0. 05),with a significant difference;atropine group in the first experiment 3,7, 14 days,compared with the previous three checks experiment (P < 0. 05),meaning the difference,and the first seven days difference between the maximum,to test the first 21 days,P > 0. 05,no significant difference;On experiment 3 day compared scopolamine group than atropine group (P < 0. 05),the difference was significant;the first 21 days the experi-mental group than in the scopolamine atropine group (P < 0. 05),the difference was righteous. Lacrimal gland pathology scopolamine group was there a large group of inflammatory cell infiltration compared with atropine,glandular epithelium at-rophy. Conclusions Atropine sulfate eye drops for dry eye model produced economic,convenient,rapid onset,but can not be sustained;scopolamine hydrobromide from subcutaneous injections produced slow onset of dry eye model,but long-term stability,and the impact of the lacrimal gland epithelial cells obvious.