CAJ | 학술논문

目的：研究超支化聚缩水甘油-羟喜树碱药物递送系统（biodegradable hyperbranched polyglycerol-hydroxy camp-tothecin, dHPG-HA）对体外培养的人腺样囊性癌（高肺转移）细胞（salivary adenoid cystic carcinoma-lung metastases, SACC-LM）的抑制作用。方法：细胞计数试剂盒（cell counting kit, CCK8）检测药物浓度为0.001、0.01、0.1、1、10、100μg/mL的羟喜树碱（hydroxy camptothecin, HA）和含等量HA的dHPG-HA，在24、48 h对肿瘤细胞的抑制作用；流式细胞仪检测浓度为0.001、0.01、0.1、1、10μg/mL的dHPG-HA是否能诱导SACC-LM细胞凋亡。结果：HA和dHPG-HA对SACC-LM细胞增殖均有明显抑制作用，且作用呈剂量依赖性和时间依赖性；dHPG-HA对SACC-LM细胞的细胞毒性明显低于HA；dHPG-HA能诱导SACC-LM细胞凋亡。结论：dHPG的构建能达到使药物HA缓释的作用并降低其细胞毒性。
목적：연구초지화취축수감유-간희수감약물체송계통（biodegradable hyperbranched polyglycerol-hydroxy camp-tothecin, dHPG-HA）대체외배양적인선양낭성암（고폐전이）세포（salivary adenoid cystic carcinoma-lung metastases, SACC-LM）적억제작용。방법：세포계수시제합（cell counting kit, CCK8）검측약물농도위0.001、0.01、0.1、1、10、100μg/mL적간희수감（hydroxy camptothecin, HA）화함등량HA적dHPG-HA，재24、48 h대종류세포적억제작용；류식세포의검측농도위0.001、0.01、0.1、1、10μg/mL적dHPG-HA시부능유도SACC-LM세포조망。결과：HA화dHPG-HA대SACC-LM세포증식균유명현억제작용，차작용정제량의뢰성화시간의뢰성；dHPG-HA대SACC-LM세포적세포독성명현저우HA；dHPG-HA능유도SACC-LM세포조망。결론：dHPG적구건능체도사약물HA완석적작용병강저기세포독성。
Objective: To investigate the inhibition effects of hyperbranched polyglycidol-hydroxy camptothecin (dHPG-HA) drug delivery system on cultured human adenoid cystic carcinoma (high lung metastasis) cells (SACC-LM). Methods:SACC-LM cells cultured in vitro were treated with different concentrations of hydroxy camptothecin (HA) and (dHPG-HA) which contained equal amounts of HA, and the proliferation inhibition of SACC-LM cells was detected with cell counting kit assay (CCK8). Apoptosis of SACC-LM cells treated by various concentrations of HA and dHPG-HA was ascertained by flow cytometry. Results:The proliferation of SACC-LM cells was significantly reduced. And the effect was dose-dependent and time-dependent. In addition, the cytotoxicity of dHPG-HA on SACC-LM cells was obviously lower than free HA. Fur-thermore, dHPG-HA could induce apoptosis of SACC-LM cells effectively. In a word, the construction of drug carrier (dH-PG) on HA provided sustained release of drugs and reduced its cytotoxicity.