CAJ | 학술논문

目的：探讨EIF5A2在结直肠癌细胞中的表达及在化疗抵抗中的作用。方法：培养对数生长期的人结直肠癌细胞系LOVO、SW480及DLD1，用Western blot法测定三种结直肠癌细胞系中EIF5A2的表达。将人结直肠癌LOVO细胞在浓度为0.2μg/L的5-Fu中培养48 h，用Westernblot法测定EIF5A2的表达。EIF5A2-siRNA转染LOVO细胞，将转染后的LOVO细胞及结直肠癌LOVO细胞在0.2μg/L的5-Fu中培养48 h，用MTT法测定细胞生长抑制率。结果：EIF5A2在三种结直肠癌细胞系中均有表达，在LOVO细胞中表达量最高（P＜0.05）。结直肠癌LOVO细胞在5-Fu中培养48 h后 EIF5A2表达明显增强（P＜0.05）。EIF-5A2-siRNA转染LOVO细胞后增加了5-Fu的细胞抑制率（P＜0.05）。结论：EIF5A2在人结直肠癌细胞中有表达，并且增强了5-Fu的化疗抵抗力。
목적：탐토EIF5A2재결직장암세포중적표체급재화료저항중적작용。방법：배양대수생장기적인결직장암세포계LOVO、SW480급DLD1，용Western blot법측정삼충결직장암세포계중EIF5A2적표체。장인결직장암LOVO세포재농도위0.2μg/L적5-Fu중배양48 h，용Westernblot법측정EIF5A2적표체。EIF5A2-siRNA전염LOVO세포，장전염후적LOVO세포급결직장암LOVO세포재0.2μg/L적5-Fu중배양48 h，용MTT법측정세포생장억제솔。결과：EIF5A2재삼충결직장암세포계중균유표체，재LOVO세포중표체량최고（P＜0.05）。결직장암LOVO세포재5-Fu중배양48 h후 EIF5A2표체명현증강（P＜0.05）。EIF-5A2-siRNA전염LOVO세포후증가료5-Fu적세포억제솔（P＜0.05）。결론：EIF5A2재인결직장암세포중유표체，병차증강료5-Fu적화료저항력。
Objective: To research EIF-5A2 overexpression in colorectal cancer and its effect on chemo-therapy.Methods: EIF5A2 expression in colorectal carcinoma (CRC) line (LOVO, SW48 and DLD1) was mea-sured by Western-blot. LOVO cells were cultured in 5-Fu for 48 hours, and EIF5A2 expression was measured by Western-blot. LOVO cells were treated with EIF5A2-siRNA and exposed to 0.2 μg/L 5-Fu for 48 hours. Cell tox-icity was assayed by MTT.Results: EIF5A2 was expressed in all three different CRC and was highest in LOVO cells (P<0.05). 5-Fu treatment led to elevated EIF5A2 expression (P<0.05). The transfection of EIF5A2-siRNA could improve the inhibit rate of LOVO to 5-Fu (P<0.05).Conclusion: EIF5A2 is expressed in CRC lines and it can enhance the chemoresistance of 5-Fu.