CAJ | 학술논문

目的：观察姜黄素对大鼠带状疱疹神经痛动物模型行为学及脊髓背角和背根神经节（DRG）的磷酸化核因子-κB（p-NF-κB）的影响，探讨姜黄素用于带状疱疹神经痛治疗的疗效及其机制。方法：选取雄性SD大鼠80只，随机分为4组（各20只）：C组、D组、Dcur1组、Dcur2组。C组：后爪注射0.9%氯化钠溶液；D组：将单纯疱疹病毒-1（HSV-1）接种于大鼠后爪，5 d后行0.9%氯化钠溶液腹腔注射治疗。Dcur1组、Dcur2组：HSV-1接种大鼠5 d后行腹腔内姜黄素注射治疗，剂量分别为100 mg/kg、200 mg/kg。于造模前、造模后5 d（治疗前）、治疗结束后1、7、14 d，测定机械缩足阈值（MWT）、热缩足潜伏期（TWL）；于治疗结束后14 d检测脊髓和DRG中p-NF-κB表达。结果：大鼠感染HSV-1后MWT从43.8降低到15.0，TWL从10.2降低到6.7，脊髓背角p-NF-κB表达从1.3增加到26.1，DRG中p-NF-κB表达从0.7增加到12.1。2组大鼠注射姜黄素后的MWT和TWL均明显增加，而p-NF-κB的表达均明显降低（Dcur1组脊髓背角和DRG分别降低到5.7和4.8，Dcur2组脊髓背角和DRG分别降低到3.1和2.0）。结论：姜黄素早期注射能明显缓解带状疱疹神经痛，其机制与下调脊髓背角和DRG的p-NF-κB过表达有关。
목적：관찰강황소대대서대상포진신경통동물모형행위학급척수배각화배근신경절（DRG）적린산화핵인자-κB（p-NF-κB）적영향，탐토강황소용우대상포진신경통치료적료효급기궤제。방법：선취웅성SD대서80지，수궤분위4조（각20지）：C조、D조、Dcur1조、Dcur2조。C조：후조주사0.9%록화납용액；D조：장단순포진병독-1（HSV-1）접충우대서후조，5 d후행0.9%록화납용액복강주사치료。Dcur1조、Dcur2조：HSV-1접충대서5 d후행복강내강황소주사치료，제량분별위100 mg/kg、200 mg/kg。우조모전、조모후5 d（치료전）、치료결속후1、7、14 d，측정궤계축족역치（MWT）、열축족잠복기（TWL）；우치료결속후14 d검측척수화DRG중p-NF-κB표체。결과：대서감염HSV-1후MWT종43.8강저도15.0，TWL종10.2강저도6.7，척수배각p-NF-κB표체종1.3증가도26.1，DRG중p-NF-κB표체종0.7증가도12.1。2조대서주사강황소후적MWT화TWL균명현증가，이p-NF-κB적표체균명현강저（Dcur1조척수배각화DRG분별강저도5.7화4.8，Dcur2조척수배각화DRG분별강저도3.1화2.0）。결론：강황소조기주사능명현완해대상포진신경통，기궤제여하조척수배각화DRG적p-NF-κB과표체유관。
Objective: To evaluate the effect of curcumin on herpetic neuralgia in rats and its mechanisms. Methods: Sixty rats with therpetic neuralgia induced by claw injection of HSV-1 and 20 healthy control (C group) rats whose claw were injected by normal saline were included. Twenty rats which had been injected HSV-1 were injected in intraperitoneal 100 mg/kg curcumin 5 days later (Dcur1 group), and 20 rats were injected 200 mg/kg curcumin (Dcur2 group), and the rest 20 rats were injected normal saline (D group ). We tested mechani-cal allodynia with electronic von frey and tested thermal hyperalgesia with radiant heat before HSV-1 injection, 5 days later after HSV-1 injection, on 1, 7, 14 days after curcumin or NS injection. p-NF-κB in lumbar spinal cord (L4~5) and DRG was estimated by immunohistochemistry at 14 d after NS or curcumine was given.Results:Compared to C group, Shingles rats exhibited signiifcant pain hypersensitivity, as evidenced by decreased paw withdrawal threshold to electronic von Frey and decreased paw withdrawal latency to heat. After herpes infec-tion, rat’s mechanical paw withdrawal threshold reduced from 43.8 to 15.0, and thermal withdrawal latencies reduced from 10.2 to 6.7. Phosphorylation of nuclear factor (p-NF)-κB expression in the spinal cord dorsal horn increased from 1.3 to 26.1, while phosphorylation of nuclear factor (p-NF)-κB expression in the dorsal root gan-glion increased from 0.7 to 12.1. However, curcumine signiifcantly attenuated the Shingles-induced allodynia and hyperalgesia and reduced the expression of p-NF-κB (Dcur1 spinal cord dorsal horn/dorsal root ganglion reduced to 5.7/4.8, Dcur2 spinal cord dorsal horn/dorsal root ganglion reduced to 3.1/2.0).Conclusion: Curcumine seems attenuate pain threshold in herpetic neuralgia rats, possibly through an inhibitory action on p-NF-κB expression in spinal dorsal horn and dorsal root ganglion.