CAJ | 학술논문

目的：通过裸鼠实验研究，证实沉默黏着斑激酶（FAK）的表达能有效抑制大肠癌的转移。方法：将24只裸鼠随机分成3组：实验组、阴性对照组、未处理组，分别注射FAK稳定沉默的结肠癌细胞、阴性对照组的结肠癌细胞、未特殊处理的结肠癌细胞，每组各8只裸鼠。在SPF条件动物实验室饲养6周后处死裸鼠，观察原位成瘤率、病死率、瘤体生长、肝肺转移情况，通过HE染色、免疫组织化学染色研究FAK微转移灶、蛋白表达情况。结果：各组成瘤率均为100%。与阴性对照组及未处理组相比，实验组转移率、病死率和FAK蛋白表达明显降低（P＜0.05），且转移灶恶变程度更低、形态更好，而阴性对照组与未处理组差异无统计学意义（P＞0.05）。结论：通过shRNA介导的FAK基因沉默对SW620原位移植后的裸鼠肝肺转移有明显抑制，FAK有望成为治疗大肠癌转移的新靶点。
목적：통과라서실험연구，증실침묵점착반격매（FAK）적표체능유효억제대장암적전이。방법：장24지라서수궤분성3조：실험조、음성대조조、미처리조，분별주사FAK은정침묵적결장암세포、음성대조조적결장암세포、미특수처리적결장암세포，매조각8지라서。재SPF조건동물실험실사양6주후처사라서，관찰원위성류솔、병사솔、류체생장、간폐전이정황，통과HE염색、면역조직화학염색연구FAK미전이조、단백표체정황。결과：각조성류솔균위100%。여음성대조조급미처리조상비，실험조전이솔、병사솔화FAK단백표체명현강저（P＜0.05），차전이조악변정도경저、형태경호，이음성대조조여미처리조차이무통계학의의（P＞0.05）。결론：통과shRNA개도적FAK기인침묵대SW620원위이식후적라서간폐전이유명현억제，FAK유망성위치료대장암전이적신파점。
Objective: Focal adhesion kinase gene silencing by Short Hairpin RNA can effectively inhibit colorectal cancer’s metastasis were conifrmed through injection of FAK-knockdown colorectal cancer cells into nude mice.Methods: Twenty-four nude mice were randomly divided into three groups which were experimental group which inject FAK-knockdown colon cancer cells, negative control group whick inject negative control colon cancer cells and untreated group which inject untreated colon cancer cells. Nude mice were sacriifced after 6 weeks and tumor formation rate, mortality, tumor growth, liver and lung’s metastasis, miscrometastasis were observed and FAK protein expression were determined with HE staining and immunohistochemical staining. Results: Tumor formation rate was 100% in each group. Distant metastases, mortality and FAK protein expres-sion of experimental group was signiifcantly lower than that of the other two groups (P<0.05), meanwhile, the malignant lesion extent was lighter. While negative control group and untreated group were not signiifcantly dif-ferent (P>0.05).Conclusion: Lentiviral packaging shRNA-mediated FAK gene silencing can signiifcantly inhibit liver and lung metastasis of colorectal cancer. FAK is expected to be a new target for treatment of colon cancer metastasis.