CAJ | 학술논문

目的：通过检测Notch 1信号通路的下游表达物Jagged 1、Hes 1在轻、中、重三种闭合性颅脑损伤（TBI）大鼠中的表达情况，探讨Notch 1信号通路在颅脑损伤中的作用。方法：利用液压冲击法模拟大鼠轻、中、重三种闭合性TBI模型；通过神经系统疾病严重程度评分（NSS）评估24 h后各组大鼠神经功能缺失情况；免疫组织化学及蛋白印迹方法检测三型TBI后脑皮质Notch 1信号通路下游表达因子Jagged 1及Hes 1的表达情况。结果：轻、中、重3组TBI模型中，NSS评分差异有统计学意义（P＜0.05），造成的脑损伤越重，神经功能缺失情况越明显，说明模型建立成功；免疫组织化学及Western blot法检测结果显示轻、中、重三型TBI组的Hes 1和Jagged 1的表达水平均较对照组明显增高（P＜0.05），且随着TBI程度的增高，该通路蛋白的表达量也在增加。结论：Notch 1信号通路可能参与了TBI的发展过程，可能成为TBI后治疗的新靶点。
목적：통과검측Notch 1신호통로적하유표체물Jagged 1、Hes 1재경、중、중삼충폐합성로뇌손상（TBI）대서중적표체정황，탐토Notch 1신호통로재로뇌손상중적작용。방법：이용액압충격법모의대서경、중、중삼충폐합성TBI모형；통과신경계통질병엄중정도평분（NSS）평고24 h후각조대서신경공능결실정황；면역조직화학급단백인적방법검측삼형TBI후뇌피질Notch 1신호통로하유표체인자Jagged 1급Hes 1적표체정황。결과：경、중、중3조TBI모형중，NSS평분차이유통계학의의（P＜0.05），조성적뇌손상월중，신경공능결실정황월명현，설명모형건립성공；면역조직화학급Western blot법검측결과현시경、중、중삼형TBI조적Hes 1화Jagged 1적표체수평균교대조조명현증고（P＜0.05），차수착TBI정도적증고，해통로단백적표체량야재증가。결론：Notch 1신호통로가능삼여료TBI적발전과정，가능성위TBI후치료적신파점。
Objective: By detecting Notch 1 signaling pathway proteins Jagged 1 and Hes 1 in light of heavy colsed craniocerebral injury. The role of Notch 1 signaling pathways in traumatic brain injury was explore and the new direction of treatment for closed craniocerebral injury was provide.Methods: Use lateral lfuid per-cussion method to make the light, medium and heavy closed craniocerebral trauma models. Groups of rats after 24 h of neurologic deifcits were evaluated through the nervous system disease severity score (NSS), then use immunohistochemistry and Western blot methods were used to detect Notch 1 signaling pathway downstream factors Jagged 1 and Hes 1.Results: The NSS score difference among the TBI groups was statistically signiifcant (P<0.05), the brain contusion was heavier, the neurologic deifcits were more obvious, which means the model was established successfully. Immunohistochemistry and Western blot showed that in brain contusion the expres-sion of Hes 1 and Jagged 1 levels were signiifcantly higher than that the control group (P<0.05), and increased with the degree of brain injury, the expression of the proteins was increased.Conclusion: Notch 1 signaling path-way may be involved in the development process of craniocerebral injury, and may become the new target for therapy after traumatic brain injury.