中国油脂
中國油脂
중국유지
CHINA OILS AND FATS
2015年
6期
57-61
,共5页
复合磷脂酶%水相体系%甘油磷脂酰胆碱%酶法制备
複閤燐脂酶%水相體繫%甘油燐脂酰膽堿%酶法製備
복합린지매%수상체계%감유린지선담감%매법제비
complex phospholipase%aqueous media%glycerylphosphorylcholine%enzymatic preparation
采用复合磷脂酶在水相体系中酶解大豆卵磷脂制备甘油磷脂酰胆碱(GPC),磷脂酶A1和磷脂酶A2复合酶解作用,不仅有效地缩短了酶解时间,更能提高GPC的得率。通过响应面优化试验确定最佳酶解条件为：底物质量浓度61 g/L,酶解温度45.2℃,磷脂酶A1添加量16.86 U/mL,磷脂酶A2添加量21.98 U/mL,CaCl2添加量5.18 g/L。在最佳酶解条件下酶解2 h,GPC得率平均值为96.25%。
採用複閤燐脂酶在水相體繫中酶解大豆卵燐脂製備甘油燐脂酰膽堿(GPC),燐脂酶A1和燐脂酶A2複閤酶解作用,不僅有效地縮短瞭酶解時間,更能提高GPC的得率。通過響應麵優化試驗確定最佳酶解條件為：底物質量濃度61 g/L,酶解溫度45.2℃,燐脂酶A1添加量16.86 U/mL,燐脂酶A2添加量21.98 U/mL,CaCl2添加量5.18 g/L。在最佳酶解條件下酶解2 h,GPC得率平均值為96.25%。
채용복합린지매재수상체계중매해대두란린지제비감유린지선담감(GPC),린지매A1화린지매A2복합매해작용,불부유효지축단료매해시간,경능제고GPC적득솔。통과향응면우화시험학정최가매해조건위：저물질량농도61 g/L,매해온도45.2℃,린지매A1첨가량16.86 U/mL,린지매A2첨가량21.98 U/mL,CaCl2첨가량5.18 g/L。재최가매해조건하매해2 h,GPC득솔평균치위96.25%。
Glycerylphosphorylcholine ( GPC) was prepared by the enzymatic hydrolysis of soybean lecithin in aqueous media with complex phospholipase as catalyst, and the complex enzymatic hydrolysis by phos-pholipase A1 and phospholipase A2 could not only effectively shorten the enzymolysis time, but also im-prove the yield of GPC. The optimal enzymatic hydrolysis conditions were determined by response surface methodology as follows:mass concentration of substrate 61 g/L, enzymolysis temperature 45. 2℃, dosage of phospholipase A1 16. 86 U/mL, dosage of phospholipase A2 21. 98 U/mL, dosage of CaCl2 5. 18 g/L. Under the optimal conditions, the average yield of GPC was 96. 25%after enzymatic hydrolysis for 2 h.
