CAJ | 학술논문

采用MTT比色法研究α-亚麻酸对 HepG2细胞的损伤作用,利用倒置相差显微镜观察HepG2细胞形态,并通过测定ROS水平、SOD活性以及MDA含量来探讨α-亚麻酸对HepG2细胞氧化应激的影响。结果表明：α-亚麻酸在50~250μmol/L浓度范围内呈剂量-时间依赖性方式抑制HepG2细胞的生长增殖,说明α-亚麻酸能够诱导HepG2细胞损伤；α-亚麻酸在200μmol/L浓度下作用24 h后HepG2细胞形态发生明显改变,并在高浓度时HepG2细胞呈典型的凋亡形态学改变；α-亚麻酸作用HepG2细胞后致使细胞大量生成ROS,SOD活性下降,MDA含量升高,这说明α-亚麻酸对HepG2细胞氧化应激产生影响,从而诱导HepG2细胞损伤。
채용MTT비색법연구α-아마산대 HepG2세포적손상작용,이용도치상차현미경관찰HepG2세포형태,병통과측정ROS수평、SOD활성이급MDA함량래탐토α-아마산대HepG2세포양화응격적영향。결과표명：α-아마산재50~250μmol/L농도범위내정제량-시간의뢰성방식억제HepG2세포적생장증식,설명α-아마산능구유도HepG2세포손상；α-아마산재200μmol/L농도하작용24 h후HepG2세포형태발생명현개변,병재고농도시HepG2세포정전형적조망형태학개변；α-아마산작용HepG2세포후치사세포대량생성ROS,SOD활성하강,MDA함량승고,저설명α-아마산대HepG2세포양화응격산생영향,종이유도HepG2세포손상。
The injury of α-linolenic acid on HepG2 cell was investigated by MTT colorimetric method, inverted phase contrast microscope was used to observe morphology of HepG2 cell and the reactive oxygen species(ROS) level, superoxide dismutase(SOD) activity and malonadialdehyde(MDA) content were determined to discuss the effect of α-linolenic acid on the oxidative stress of HepG2 cell. The results showed that the proliferation of HepG2 cell was inhibited by α-linolenic acid in a dosage-dependent (50-250 μmol/L) and time-dependent manner, which indicated that α-linolenic acid could induce the injury of HepG2 cell. The morphology of HepG2 cell changed after incubated with 200 μmol/L α-linolenic acid for 24 h and showed typical apoptotic morphology changes in high concentration. After treatment with α-linolenic acid,the contents of ROS and MDA in HepG2 cell increased,while the activi-ty of SOD decreased, indicating that α-linolenic acid affected the oxidative stress of HepG2 cell to in-duce the injury of HepG2 cell.