CAJ | 학술논문

以棉籽贮存蛋白为原料，采用蛋白纯化系统(配备凝胶层析柱)对棉籽7 S 和12 S球蛋白进行分离纯化，对所得7 S和12 S球蛋白的蛋白质含量、氨基酸组成、变性温度及荧光强度等物化性质进行了测定分析。结果表明：棉籽7 S 及12 S 球蛋白的蛋白质含量分别为(91．59±0．53)%和(93．35±0．62)%，其SDS-PAGE谱图中除显示各自特征条带外无其他杂带，其氨基酸组成与绝大部分油料种子中球蛋白的基本一致，含有丰富的谷氨酸、天冬氨酸和精氨酸，而半胱氨酸和甲硫氨酸含量较低；差示扫描量热仪( DSC )分析棉籽7 S及12 S球蛋白的变性温度分别为93．3℃和107．7℃；棉籽7 S及12 S球蛋白的荧光发射光谱最大荧光强度均在325 nm左右。
이면자저존단백위원료，채용단백순화계통(배비응효층석주)대면자7 S 화12 S구단백진행분리순화，대소득7 S화12 S구단백적단백질함량、안기산조성、변성온도급형광강도등물화성질진행료측정분석。결과표명：면자7 S 급12 S 구단백적단백질함량분별위(91．59±0．53)%화(93．35±0．62)%，기SDS-PAGE보도중제현시각자특정조대외무기타잡대，기안기산조성여절대부분유료충자중구단백적기본일치，함유봉부적곡안산、천동안산화정안산，이반광안산화갑류안산함량교저；차시소묘량열의( DSC )분석면자7 S급12 S구단백적변성온도분별위93．3℃화107．7℃；면자7 S급12 S구단백적형광발사광보최대형광강도균재325 nm좌우。
Cottonseed 7 S and 12 S globulins were isolated and purified from cottonseed storage protein by protein purification system coupled with gel chromatography,and the protein contents, amino acid compo-sitions, denaturation temperatures and fluorescence intensities of 7S and 12S globulins obtained were de-termined and analyzed. The results showed that the protein contents of cottonseed 7S and 12S globulins were (91. 59 ± 0. 53)% and (93. 35 ± 0. 62)% respectively, and there was no impurity band existed in the SDS - PAGE chromatography of cottonseed 7 S and 12 S globulins except for the characteristic bands;cottonseed 7S and 12S globulins were rich in Glu, Asp and Arg, and poor in Cys and Met, which was similar to that of most oil seeds;the differential scanning calorimetry ( DSC) results indicated that the denaturation temperatures of cottonseed 7S and 12S globulins were 93. 3℃ and 107. 7℃, respectively;and the maximum fluorescence intensities of cottonseed 7S and 12S globulins were both at about 325 nm.