CAJ | 학술논문

目的：对比微孔板法和传统方法分光光度法测定花菇、猴头菇和茶树菇体外抗氧化活性的差异。方法超声波法提取3种菇的水提物和醇提物，以VC为对照品，均与底物1，1-二苯基-2-三硝基苯肼自由基（DPPH·）反应，其中微孔板法为微孔板结合酶标仪于517 nm波长处快速测定菇类提取物的DPPH·抑制率，传统分光光度法于紫外-可见分光光度计517 nm波长处检测。对2种方法的测定结果进行曲线拟合，并进行方法重复性对比。结果2种方法抗氧化结果IC50基本一致，曲线拟合度较好，差异无统计学意义（P>0.05）；其中对照品VC的微孔板法拟合模型公式为y=23.872Lnx+152.526，分光光度法模型公式为y=22.469Lnx+146.044，2种方法的模型中系数和常数经t检验差异有统计学意义（P<0.01），提示重叠率高。分别对3种样品的提取液进行曲线拟合，结果显示3种菇提取液2种抗氧化活性测定结果的曲线拟合度均较好，差异有统计学意义（P<0.05）。实验中微孔板法试剂用量较小，精密度更高。结论2种菇类体外抗氧化活性测定方法实验结果一致，差异不明显，微孔板法在试剂用量和精密度方面略具优势。
목적：대비미공판법화전통방법분광광도법측정화고、후두고화다수고체외항양화활성적차이。방법초성파법제취3충고적수제물화순제물，이VC위대조품，균여저물1，1-이분기-2-삼초기분정자유기（DPPH·）반응，기중미공판법위미공판결합매표의우517 nm파장처쾌속측정고류제취물적DPPH·억제솔，전통분광광도법우자외-가견분광광도계517 nm파장처검측。대2충방법적측정결과진행곡선의합，병진행방법중복성대비。결과2충방법항양화결과IC50기본일치，곡선의합도교호，차이무통계학의의（P>0.05）；기중대조품VC적미공판법의합모형공식위y=23.872Lnx+152.526，분광광도법모형공식위y=22.469Lnx+146.044，2충방법적모형중계수화상수경t검험차이유통계학의의（P<0.01），제시중첩솔고。분별대3충양품적제취액진행곡선의합，결과현시3충고제취액2충항양화활성측정결과적곡선의합도균교호，차이유통계학의의（P<0.05）。실험중미공판법시제용량교소，정밀도경고。결론2충고류체외항양화활성측정방법실험결과일치，차이불명현，미공판법재시제용량화정밀도방면략구우세。
Objective To compare the determination results about microplate method and traditional spectrophotometric method on the antioxidant activity in vitro of Flower Mushroom,Hericium and Agrocybe Aegerita. Methods Ultrasonic extraction was used to extract the aqueous extracts and ethanol extracts of 3 mushrooms,VC was used as the reference substance,and given the reaction with the substrate 1,1-diphenyl-2-picrylhydrazyl radical 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl free radical (DPPH·).Microplate method was that using microplate combined with microplate reader in 517 nm wavelength for determination of DPPH·inhibition ratio of mushroom extracts fastly,traditional spec-trophotometric method was that determining in the UV Vis spectrophotometry in 517 nm wavelength for determination. Then the curve fitting and method repeated comparison was given,and method repeatability comparison was done. Re-sults Two methods of antioxidant results IC50 were consistent relatively,curve fitting was better,and no statistical differ-ence (P>0.05); microplate method fitting model formula of reference substance VC was y=23.872Lnx+152.526,spec-trophotometry methed was y=22.469Lnx+146.044,the model of the two methods’ coefficients and constants was signifi-cant (P<0.01) by the t-test,with high overlapping rate.Then respectively curve fitting on extracts of three kinds samples, the results showed that the determination results of two method curve fitting were good of three mushrooms extracts’.