世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
6期
841-843,848
,共4页
韩冰冰%卢岩%赵海军%王媛
韓冰冰%盧巖%趙海軍%王媛
한빙빙%로암%조해군%왕원
电针%MCAO%小胶质细胞%TLR4%NF-κB
電針%MCAO%小膠質細胞%TLR4%NF-κB
전침%MCAO%소효질세포%TLR4%NF-κB
Electroacupuncture%MCAO%Microglia%TLR4%NF - κB
目的:研究电针对MCAO大鼠脑组织小胶质细胞TLR4及NF-κB蛋白表达的影响。方法建立MCAO大鼠模型,分别电针内关、曲池后进行神经体征评分,HE染色检测神经元坏死率,双重免疫荧光染色检测TLR4、NF-κB p65蛋白在小胶质细胞的表达。结果模型组与正常组比较,大鼠神经体征评分、脑海马神经元坏死率、脑组织TLR4、NF-κB p65蛋白表达均明显升高,差异有统计学意义( P<0,01);内关组、曲池组与模型组比较,神经体征评分、脑海马神经元坏死率、TLR4、NF-κB p65蛋白表达明显降低,差异有统计学意义( P<0,05);曲池组与地机组比较,脑海马神经元坏死率明显降低,差异有统计学意义( P<0,05);内关组与地机组比较,大鼠脑组织小胶质细胞TLR4蛋白表达明显降低,差异有统计学意义( P<0,05)。结论电针内关、曲池能改善MCAO大鼠的神经功能损伤,抑制TLR4及NF-κB蛋白在小胶质细胞的表达,提示电针能够通过影响小胶质细胞TLR4/NF-κB信号通路的活化从而发挥脑保护作用。
目的:研究電針對MCAO大鼠腦組織小膠質細胞TLR4及NF-κB蛋白錶達的影響。方法建立MCAO大鼠模型,分彆電針內關、麯池後進行神經體徵評分,HE染色檢測神經元壞死率,雙重免疫熒光染色檢測TLR4、NF-κB p65蛋白在小膠質細胞的錶達。結果模型組與正常組比較,大鼠神經體徵評分、腦海馬神經元壞死率、腦組織TLR4、NF-κB p65蛋白錶達均明顯升高,差異有統計學意義( P<0,01);內關組、麯池組與模型組比較,神經體徵評分、腦海馬神經元壞死率、TLR4、NF-κB p65蛋白錶達明顯降低,差異有統計學意義( P<0,05);麯池組與地機組比較,腦海馬神經元壞死率明顯降低,差異有統計學意義( P<0,05);內關組與地機組比較,大鼠腦組織小膠質細胞TLR4蛋白錶達明顯降低,差異有統計學意義( P<0,05)。結論電針內關、麯池能改善MCAO大鼠的神經功能損傷,抑製TLR4及NF-κB蛋白在小膠質細胞的錶達,提示電針能夠通過影響小膠質細胞TLR4/NF-κB信號通路的活化從而髮揮腦保護作用。
목적:연구전침대MCAO대서뇌조직소효질세포TLR4급NF-κB단백표체적영향。방법건립MCAO대서모형,분별전침내관、곡지후진행신경체정평분,HE염색검측신경원배사솔,쌍중면역형광염색검측TLR4、NF-κB p65단백재소효질세포적표체。결과모형조여정상조비교,대서신경체정평분、뇌해마신경원배사솔、뇌조직TLR4、NF-κB p65단백표체균명현승고,차이유통계학의의( P<0,01);내관조、곡지조여모형조비교,신경체정평분、뇌해마신경원배사솔、TLR4、NF-κB p65단백표체명현강저,차이유통계학의의( P<0,05);곡지조여지궤조비교,뇌해마신경원배사솔명현강저,차이유통계학의의( P<0,05);내관조여지궤조비교,대서뇌조직소효질세포TLR4단백표체명현강저,차이유통계학의의( P<0,05)。결론전침내관、곡지능개선MCAO대서적신경공능손상,억제TLR4급NF-κB단백재소효질세포적표체,제시전침능구통과영향소효질세포TLR4/NF-κB신호통로적활화종이발휘뇌보호작용。
Objective To investigate the effects of electroacupuncture on the protein expression of TLR4 and NF - κB p65 in microglia cells in cerebral of middle cerebral artery occlusion( MCAO)Rats, Methods After electroacupuncture on Neiguan(PC6)and Quch(i LI11)respectively,we observed neurological syndrome score of MCAO rats, The death rate of neuron were measured by HE dyeing,and the protein expression of TLR4 and NF - κB p65 in microglia cells were detected by doubling immunofluorescent staining, Results Compared to sham - operation control group,neurological syndrome score,death rate of neuron and the protein expression of TLR4 and NF - κB p65 in microglia cells were increased in model group( P < 0, 01), Compared to model group,neurological syndrome score,death rate of neuron,the protein expression of TLR4 and NF - κB p65 in microglia cells were decreased in Neiguan and Quchi group(P <0, 05), Compared to Diji group,the death rate of neuron were decreased in Quchi group(P <0, 05),and the protein expression of TLR4 in microglia cells were decreased in Neiguan group(P <0, 05), Conclusion After electroacupuncture on Neiguan and Quchi,the damage of cerebral nerve function of MCAO rats are improved,and the expression of TLR4 and NF - κB p65 in microglia cells are inhibited, It indicates that through effects on the activation of TLR4/ NF - κB signal pathway in microglia cells,electroacupuncture can play a protective role on cerebral ischemia.