CAJ | 학술논문

目的：探索建立符合中医病证特点的上焦水饮内停大鼠模型，并进行生物学特征研究。方法将40只大鼠随机分为空白对照组和模型组，每组20只。模型组给予异丙肾上腺素（ISO）腹部皮下注射，第1天20 mg/ kg、第2天10 mg/ kg、第3天5 mg/ kg、从第4天开始3 mg/ kg，1次/ d，连续17 d，观察2周，空白对照组腹部皮下注射等量生理盐水。第34天开始模型组行气管插管手术，术后寒冷刺激7 d。结果与空白对照组比较，模型组大鼠肺通透指数明显升高，差异有统计学意义（ P<0.05）；肺泡灌洗液回抽量明显升高，差异有统计学意义（P <0.05）；肺干湿比明显升高，差异有统计学意义（P <0.05）；心脏器重量及脏器指数显著升高，差异有统计学意义（P <0,01）、肺脏器重量及脏器指数有上升趋势；肌酸激酶（CK）表达明显升高，差异有统计学意义（P <0,05）；模型组大鼠 HE染色显示肺泡壁结构异常，多数肺泡腔内充满粉红色蛋白性液体，呈均质状；部分心肌细胞有嗜酸性变性和（或）坏死的病理改变。结论实验结果表明，上焦水饮内停证动物模型造模方法简单、客观，符合中医病症特点。
목적：탐색건립부합중의병증특점적상초수음내정대서모형，병진행생물학특정연구。방법장40지대서수궤분위공백대조조화모형조，매조20지。모형조급여이병신상선소（ISO）복부피하주사，제1천20 mg/ kg、제2천10 mg/ kg、제3천5 mg/ kg、종제4천개시3 mg/ kg，1차/ d，련속17 d，관찰2주，공백대조조복부피하주사등량생리염수。제34천개시모형조행기관삽관수술，술후한랭자격7 d。결과여공백대조조비교，모형조대서폐통투지수명현승고，차이유통계학의의（ P<0.05）；폐포관세액회추량명현승고，차이유통계학의의（P <0.05）；폐간습비명현승고，차이유통계학의의（P <0.05）；심장기중량급장기지수현저승고，차이유통계학의의（P <0,01）、폐장기중량급장기지수유상승추세；기산격매（CK）표체명현승고，차이유통계학의의（P <0,05）；모형조대서 HE염색현시폐포벽결구이상，다수폐포강내충만분홍색단백성액체，정균질상；부분심기세포유기산성변성화（혹）배사적병리개변。결론실험결과표명，상초수음내정증동물모형조모방법간단、객관，부합중의병증특점。
Objective To explore and prepare the rat model of harmful fluid retention in the upper jiao in compliance with the syndrome characteristics of TCM and have biological characteristics study, Meth-ods Forty rats were randomized into a blank group and a model group,20 rats in each one, In the model group,isopropyl epinephrine(ISO)was injected subcutaneously in the abdomen,20 mg/ kg on the first day, 10mg/ kg on the second day and 5 mg/ kg on the third day;3 mg/ kg since the forth day,once a day,continu-ously for 17 days, The observation lasted for 2 weeks, In the blank control group,the physical saline of the same dose was injected subcutaneously in the abdomen, The endotracheal intubation was done since the 34th day in the model group, After operation,the cold irritation was given for 7 days, Results Compared with the blank control group,the lung permeability index was increased apparently in the model group,indicating the significant difference(P < 0, 05);the pulmonary alveolus lavage pumpback amount was increased apparently, indicating the significant difference(P < 0, 05);the pulmonary dry wet ratio was increased apparently,indica-ting the significant difference(P < 0, 05);the weight of heart and organ index were increased significantly, presenting the significant difference(P < 0, 01)and the weight of lung and organ index was increased in tend-ency, CK expression was increased apparently,indicating the significant difference(P < 0, 05), In the model group,HE staining indicated that the structure of alveolar wall was abnormal,and most of the alveolar cavities were filled with pink protein - like liquid and homogeneous in nature, A part of the myocardial cells presented eosinophilic degeneration and/ or necrosis in pathology, Conclusion The experiment results show that the animal modeling method of harmful fluid retention in the upper jiao is simple and objective and is in compli-ance with the pathological characteristics of TCM.