CAJ | 학술논문

目的：探讨姜黄素介导光动力疗法（Photodynamic therapy，PDT）对裸鼠宫颈癌移植瘤模型的影响及其可能机制。方法将宫颈癌 Me -180细胞悬液注入裸鼠皮下构建移植瘤模型，随机分为4组：模型组、PDT 组、姜黄素组、姜黄素+ PDT 组，每组12只。各组治疗1 d 后处死6只，剩余6只分别在第1、3、7、14、21天测量肿瘤体积。制备病理切片，观察各组 HE 病理改变、免疫组化。West-en - blot 检测肿瘤组织中 Notch -1蛋白的表达情况。结果模型组肿瘤体积逐渐增大，其余各组肿瘤体积在14 d 内无增大趋势，随后逐渐增大。姜黄素+ PDT 组 Notch -1蛋白表达与模型组比较，差异有统计学意义（P <0,01）。结论姜黄素介导的 PDT 能够有效抑制宫颈癌体外移植瘤的增长，其机制可能通过 Notch -1信号通路发挥作用。
목적：탐토강황소개도광동력요법（Photodynamic therapy，PDT）대라서궁경암이식류모형적영향급기가능궤제。방법장궁경암 Me -180세포현액주입라서피하구건이식류모형，수궤분위4조：모형조、PDT 조、강황소조、강황소+ PDT 조，매조12지。각조치료1 d 후처사6지，잉여6지분별재제1、3、7、14、21천측량종류체적。제비병리절편，관찰각조 HE 병리개변、면역조화。West-en - blot 검측종류조직중 Notch -1단백적표체정황。결과모형조종류체적축점증대，기여각조종류체적재14 d 내무증대추세，수후축점증대。강황소+ PDT 조 Notch -1단백표체여모형조비교，차이유통계학의의（P <0,01）。결론강황소개도적 PDT 능구유효억제궁경암체외이식류적증장，기궤제가능통과 Notch -1신호통로발휘작용。
Objective To observe the effect of curcumin mediate Photodynamic therapy(PDT)on xenografted tumor of human cervical cancer and explore its mechanisms, Methods Human cervical cancer cells(Me - 180)were implanted in the back of the Bal/ bc nude mice, After filtration,all mice were randomly divided into 4 groups,including model group,PDT group,curcumin group,and curcumin + PDT group, There were12 mice in each group, Six rats were executed one day after the treatment and the remaining 6 rats were left to measure the volume of the tumor on the 1st ,3rd ,7th ,14th and 21st day, The pathological change of each group was observed by HE staining,immunohistochemistry, Western - blot was used to detect the expression of Notch - 1 protein, Results The tumor volume of model group increased gradually,while the other 3 groups had no changes in 14 days and then increased gradually, Compared with the model group,the Notch -1 expression in the group of curcumin + PDT decreased significantly(P < 0, 01), Conclusion Curcumin me-diated PDT can effectively suppress the growth of cervical cancer transplanted tumor in vitro,and Notch - 1 signal pathway may play an important role in it.