世界中西医结合杂志
世界中西醫結閤雜誌
세계중서의결합잡지
WORLD JOURNAL OF TRADITIONAL CHINESE AND WESTERN MEDICINE
2015年
6期
785-787
,共3页
反相高效液相色谱法%表柔比星%血药浓度
反相高效液相色譜法%錶柔比星%血藥濃度
반상고효액상색보법%표유비성%혈약농도
Reversed Phase High Performance Liquid Chromatography%Epirubicin%Plasma Concen-tration
目的：建立反相高效液相色谱法测定大鼠体内表柔比星血药浓度的方法。方法色谱柱为 Agilent tc - C18；流动相为：0,01 moL/ L 磷酸二氢铵缓冲溶液（冰醋酸调 pH =4,4）：乙腈=79：21；检测波长：254 nm；流速：1,0 ml/ min；进样量：20μl；柱温：室温。选择柔红霉素作为内标，以蛋白质沉淀法提取血浆样品。结果检测表柔比星血药浓度在0,01172~10,0μg/ ml 范围内线性关系良好（r =0,9995），最低定量限为0,01172μg/ ml（S/ N≥10）；日内 RSD <5,75%，日间 RSD <4,8%；样品平均回收率为96,3%，RSD 为4.8%（n =5）；表柔比星血浆样品在72 h 内不同条件下基本稳定。结论反相高效液相色谱法测定大鼠体内表柔比星的血药浓度准确可靠，灵敏度高、专属性好，可用于表柔比星血药浓度测定。
目的：建立反相高效液相色譜法測定大鼠體內錶柔比星血藥濃度的方法。方法色譜柱為 Agilent tc - C18；流動相為：0,01 moL/ L 燐痠二氫銨緩遲溶液（冰醋痠調 pH =4,4）：乙腈=79：21；檢測波長：254 nm；流速：1,0 ml/ min；進樣量：20μl；柱溫：室溫。選擇柔紅黴素作為內標，以蛋白質沉澱法提取血漿樣品。結果檢測錶柔比星血藥濃度在0,01172~10,0μg/ ml 範圍內線性關繫良好（r =0,9995），最低定量限為0,01172μg/ ml（S/ N≥10）；日內 RSD <5,75%，日間 RSD <4,8%；樣品平均迴收率為96,3%，RSD 為4.8%（n =5）；錶柔比星血漿樣品在72 h 內不同條件下基本穩定。結論反相高效液相色譜法測定大鼠體內錶柔比星的血藥濃度準確可靠，靈敏度高、專屬性好，可用于錶柔比星血藥濃度測定。
목적：건립반상고효액상색보법측정대서체내표유비성혈약농도적방법。방법색보주위 Agilent tc - C18；류동상위：0,01 moL/ L 린산이경안완충용액（빙작산조 pH =4,4）：을정=79：21；검측파장：254 nm；류속：1,0 ml/ min；진양량：20μl；주온：실온。선택유홍매소작위내표，이단백질침정법제취혈장양품。결과검측표유비성혈약농도재0,01172~10,0μg/ ml 범위내선성관계량호（r =0,9995），최저정량한위0,01172μg/ ml（S/ N≥10）；일내 RSD <5,75%，일간 RSD <4,8%；양품평균회수솔위96,3%，RSD 위4.8%（n =5）；표유비성혈장양품재72 h 내불동조건하기본은정。결론반상고효액상색보법측정대서체내표유비성적혈약농도준학가고，령민도고、전속성호，가용우표유비성혈약농도측정。
Objective To establish the method for the determination of in vivo epirubicin deter-mined with reversed phase high performance liquid chromatography(RH - HPLC)in rats, Methods Chrom-atographic column:Agilent tc - C18 ,mobile phase:the ratio of 0, 01 mol/ L ammonium dihydrogen phosphate buffer solution(mixed with glacial acetic acid pH = 4, 4)and acetonitrile was 79: 21;detection wavelength:254 nm;flow rate:1, 0 ml/ min;sample size:20 μl;column temperature:room temperature, Daunorubicin was selected as internal standard, The protein precipitation method was used for the extraction of plasma sample, Results In the range of 0, 01172 to 10, 0μg/ ml,the plasma concentration of epirubicin presented the good linear relationship(r = 0, 999 5),and the minimum quantitative limit was 0, 011 72 μg/ ml(S/ N≥10), The intraday RSD was < 5, 75% and the daytime RSD was < 4, 8% , The average recovery of sample was 96, 3% ,RSD = 4, 8%(n = 5), In 72 h,the plasma sample of epirubicin was basically stable under different conditions, Conclusion RH - HPLC is accurate,reliable,highly sensitive and well specific in the determi-nation of plasma concentration of epirubicin in the rats in vivo, This method is applicable for the determina-tion of plasma concentration of epirubicin.
