CAJ | 학술논문

目的：分析血管紧张素Ⅱ（ AngⅡ）对人血管内皮细胞和猪血管内皮细胞的活性氧和线粒体功能的影响。方法体外培养血管内皮细胞ECV-304和PAE，用不同浓度AngⅡ处理细胞，采用流式细胞术分析细胞内活性氧、线粒体膜电位和细胞凋亡。结果血管内皮细胞内的ROS水平随AngⅡ浓度增加而增加，AngⅡ导致线粒体功能损伤和凋亡。结论血管紧张素以浓度依赖的方式导致内皮细胞活性氧水平增加，并参与血管内皮细胞的损伤。
목적：분석혈관긴장소Ⅱ（ AngⅡ）대인혈관내피세포화저혈관내피세포적활성양화선립체공능적영향。방법체외배양혈관내피세포ECV-304화PAE，용불동농도AngⅡ처리세포，채용류식세포술분석세포내활성양、선립체막전위화세포조망。결과혈관내피세포내적ROS수평수AngⅡ농도증가이증가，AngⅡ도치선립체공능손상화조망。결론혈관긴장소이농도의뢰적방식도치내피세포활성양수평증가，병삼여혈관내피세포적손상。
Objective To investigate the effect of AngiotensinⅡ( AngⅡ) on reactive oxygen species and mitochon-drial function in Human vascular endothelial cells and pig vascular endothelial cells .Methods After ECV-304 and PAE cells were treated with different concentrations of AngⅡ for different durations , reactive oxygen species , mito-chondrial membrane potential and cell apoptosis were detected by flow cytometry .Results AngⅡinduced apoptosis of ECV-304 and PAE cells.Treatment with AngⅡreduced cell membrane potential .Moreover,AngⅡtreatment signifi-cantly increased the level of reactive oxygen species in ECV-304 and PAE cells.Conclusion AngⅡsignificantly in-duces apoptosis of vascular endothelial cells possibly by increasing the reactive oxygen species level .