CAJ | 학술논문

目的：探讨硫酸化寡聚多糖PI－88对食管鳞癌细胞株TE－13增殖和细胞侵袭行为的影响。方法培养中的TE－13细胞分为A组、B组和C组。 A组用15μg? mL－1 PI－88处理，B组用30μg? mL－1 PI－88处理；C组用等体积0．9％氯化钠处理。用噻唑蓝（MTT）比色法检测0．5，1，2，4，8，16，32μg? mL－1 PI－88对TE－13细胞增殖活性的影响。用Matrigel侵袭试验研究检测15或30μg? mL－1 PI－88处理后对食管鳞癌细胞株 TE －13细胞侵袭力的影响。结果7个浓度组PI－88作用于TE－13细胞72h，各组细胞存活抑制率差异无统计学意义（P＞0．05）。 Matrigel侵袭实验结果：A、B、C 3组侵袭细胞数差异有统计学意义（P＜0．01），各组间比较差异均有统计学意义（A vs B，P＜0．05；B vs C，P＜0．01；A vs C，P＜0．01）。结论 PI－88在一定浓度范围内对TE－13细胞生长无明显影响，但可抑制TE－13细胞侵袭能力，并且其抑制作用随剂量增大而增强。
목적：탐토류산화과취다당PI－88대식관린암세포주TE－13증식화세포침습행위적영향。방법배양중적TE－13세포분위A조、B조화C조。 A조용15μg? mL－1 PI－88처리，B조용30μg? mL－1 PI－88처리；C조용등체적0．9％록화납처리。용새서람（MTT）비색법검측0．5，1，2，4，8，16，32μg? mL－1 PI－88대TE－13세포증식활성적영향。용Matrigel침습시험연구검측15혹30μg? mL－1 PI－88처리후대식관린암세포주 TE －13세포침습력적영향。결과7개농도조PI－88작용우TE－13세포72h，각조세포존활억제솔차이무통계학의의（P＞0．05）。 Matrigel침습실험결과：A、B、C 3조침습세포수차이유통계학의의（P＜0．01），각조간비교차이균유통계학의의（A vs B，P＜0．05；B vs C，P＜0．01；A vs C，P＜0．01）。결론 PI－88재일정농도범위내대TE－13세포생장무명현영향，단가억제TE－13세포침습능력，병차기억제작용수제량증대이증강。
Objective To explore the inhibition effects of sulfated oligo-saccharides PI-88, which a kind of new antitumor drug, to the prolife-ration and invasive ability of esophageal cancer cell line TE -13. Methods Cultured human esophageal cancer cell line TE-13 cell was divided into 3 groups:group A ( 15 μg? mL-1 PI-88 treated group ) , group B ( 30 μg? mL-1 PI -88 treated group ) and group C ( control group).PI-88 was prepared as 1 μg? mL-1 and 2μg? mL-1 solution with sterile saline, then it was added into transwell cell with the same volume.The last PI-88 concentration was 15 or 30μg? mL-1 in A or B group.The same volume 0.9% NaCI was added in group C as blank control.Cell proliferation ability was tested by Methylthiazolyldiphenyl-tetrazolium bromide ( MTT ) assay.Matrigel tumor invasion assay was used to evaluate the TE-13 cell invasive ability.Results TE-13 cells were cultured for 72h with different concentration PI-88, there was no significant difference in all of seven concentration groups ( P>0.05 ) . The invasive cells in Matrigel tumor invasion assay were significance difference among A, B and C groups ( P<0.01).There was significance difference in each two groups comparison ( A vs B, P <0.05;B vs C, P<0.01;A vs C, P <0.01 ) .Conclusion In certain concentrations,TE-13 cell proliferation ability was not affected by different concentration PI-88.But PI-88 could inhibit TE-13 cell invasive ability with a dose-independent effect.