CAJ | 학술논문

目的：探究胆石六号汤剂对胆色素结石豚鼠胆囊肿瘤坏死因子－α（ TNF－α）及核转录因子（ NF－κB）表达的影响。方法将雌性豚鼠40只随机分成3组，空白组10只，模型组15只，实验组15只。空白组除外，其余2组均采用饮食诱导法，建立胆色素结石模型。实验组，灌胃胆石六号汤剂7．7 g? kg－1? d－1，其余2组均灌胃用同体积的0．9％氯化钠。30 d后，观察各组豚鼠成石率，用免疫组织化学技术检测胆囊TNF－α及NF－κB蛋白表达和胆囊病理组织的改变情况。结果模型组成石率为90．62％，明显高于空白组26．76％，表明造模成功；实验组成石率为11．00％，明显低于模型组90．62％，可见药物具有抗胆结石的作用。与空白组比较，模型组的TNF－α、NF－κB蛋白表达量明显增加（P＜0．01）。与模型组比较，实验组的TNF－α、NF－κB蛋白表达量明显较少（P＜0．05）。结论胆石六号汤剂可以明显减少胆色素结石豚鼠的成石率，这可能与药物降低胆色素结石豚鼠胆组织TNF－α、NF－κB蛋白的表达有关。
목적：탐구담석륙호탕제대담색소결석돈서담낭종류배사인자－α（ TNF－α）급핵전록인자（ NF－κB）표체적영향。방법장자성돈서40지수궤분성3조，공백조10지，모형조15지，실험조15지。공백조제외，기여2조균채용음식유도법，건립담색소결석모형。실험조，관위담석륙호탕제7．7 g? kg－1? d－1，기여2조균관위용동체적적0．9％록화납。30 d후，관찰각조돈서성석솔，용면역조직화학기술검측담낭TNF－α급NF－κB단백표체화담낭병리조직적개변정황。결과모형조성석솔위90．62％，명현고우공백조26．76％，표명조모성공；실험조성석솔위11．00％，명현저우모형조90．62％，가견약물구유항담결석적작용。여공백조비교，모형조적TNF－α、NF－κB단백표체량명현증가（P＜0．01）。여모형조비교，실험조적TNF－α、NF－κB단백표체량명현교소（P＜0．05）。결론담석륙호탕제가이명현감소담색소결석돈서적성석솔，저가능여약물강저담색소결석돈서담조직TNF－α、NF－κB단백적표체유관。
Objective To study the effect of Danshiliuhao decoction on the expression of tumor necrosis factor -α( TNF -α) and nuclear factor-κB( NF -κB ) in the bile pigment with stone in Guinea pigs. Methods The female Guinea pigs were randomly divided into three groups.The blank group ( n =10 ) , the model group ( n =15 ) and test group ( Danshiliuhao,n=15 ) .The latter two groups of the Guinea pigs were both modeled as the bile pigment stones model by feeding them with a different diet .The Guinea pigs in test group were given Danshi-liuhao decoction 7.7 g? kg-1? d-1 for 30 days.The rate of stone formation and the expression of the gallbladder TNF-αand NF-κB were observed af-ter administration by immunohistochemistry.Results The rate of stone formation was 90.62% in model group, significantly higher than that (26.76%) in the blank group.The results suggested that the model for Danshiliuhao is successfully built and the drug is an effective agent for anti-gallstones.The expression of the TNF -αand NF -κB in test group were significantly lower than model group. The difference was statistically significant ( P<0.05 ) .Conclusion Danshiliuhao Decoc-tion significantly reduced the rate of pigment stone formation in Guinea pigs.This may be related to the decreased expression of TNF-αand NF-κB proteins in gallbladders of Guinea pigs by the drug.