中外医疗
中外醫療
중외의료
CHINA FOREIGN MEDICAL TREATMENT
2015年
17期
45-47
,共3页
P4%双氧水%SH-SY5Y细胞%Hoechst33258染色
P4%雙氧水%SH-SY5Y細胞%Hoechst33258染色
P4%쌍양수%SH-SY5Y세포%Hoechst33258염색
P4%H2O2%SH-SY5Y cells%Hoechst33258 staining
目的：研究P4对双氧水诱导SH-SY5Y细胞凋亡的影响。方法体外培养SH-SY5Y细胞，建立双氧水诱导SH-SY5Y细胞凋亡的模型，给予P4进行干预。应用Hoechst染色，测定细胞凋亡率。结果10uM的双氧水24 h可以使SH-SY5Y细胞凋亡率达到(56.33±13.17)%。然而，给予P4后，SH-SY5Y细胞的凋亡率明显减低,P4加双氧水组与双氧水组对比差异有统计学意义(P<0.05)。结论P4对SH-SY5Y细胞的凋亡有保护作用。
目的：研究P4對雙氧水誘導SH-SY5Y細胞凋亡的影響。方法體外培養SH-SY5Y細胞，建立雙氧水誘導SH-SY5Y細胞凋亡的模型，給予P4進行榦預。應用Hoechst染色，測定細胞凋亡率。結果10uM的雙氧水24 h可以使SH-SY5Y細胞凋亡率達到(56.33±13.17)%。然而，給予P4後，SH-SY5Y細胞的凋亡率明顯減低,P4加雙氧水組與雙氧水組對比差異有統計學意義(P<0.05)。結論P4對SH-SY5Y細胞的凋亡有保護作用。
목적：연구P4대쌍양수유도SH-SY5Y세포조망적영향。방법체외배양SH-SY5Y세포，건립쌍양수유도SH-SY5Y세포조망적모형，급여P4진행간예。응용Hoechst염색，측정세포조망솔。결과10uM적쌍양수24 h가이사SH-SY5Y세포조망솔체도(56.33±13.17)%。연이，급여P4후，SH-SY5Y세포적조망솔명현감저,P4가쌍양수조여쌍양수조대비차이유통계학의의(P<0.05)。결론P4대SH-SY5Y세포적조망유보호작용。
Objective To study the effects of P4 on hydrogen peroxide induced apoptosis in SH-SY5Y cells. Methods Model of hydrogen peroxide induced apoptosis in SH-SY5Y cells which were cultured in vitro was built, for the intervention for which, P4 was used. The ratio of apoptosis determined by hoechst staining. Results Apoptosis rate of SH-SY5Y cell was 56.33% ± 13.17%under 10uM hydrogen peroxide in 24 hours. However, after giving P4, SH-SY5Y cell apoptosis rate was significantly reduced. There were statistically significant differences between P4 plus hydrogen peroxide and hydrogen peroxide group (P<0.05). Conclu-sion P4 on apoptosis of SH-SY5Y cells have a protective effect.
