CAJ | 학술논문

目的：观察人脐静脉内皮细胞（ HuVECs ）分泌物对自身迁移和侵袭的影响。方法将传代的HuVECs分为3组，A、B组分别加入一定浓度的FBS及HuVECs培养24、48 h的上清液，C组加一定浓度的FBS及纯DMEM培养液。采用细胞划痕实验观察细胞迁移距离，采用 Transwell 迁移和侵袭实验观察细胞迁移和侵袭个数。结果 A、B、C组迁移距离分别为（219．27±28．44）、（163．64±19．67）、（158．18±16．36）μm；A组与B、C组比较， P均＜0．05；B、C组比较，P＞0．05。 A组迁移细胞数多于B、C组（P均＜0．01），B、C组迁移细胞数无明显差异（P＞0．05）；A组侵袭细胞数少于B组，B组少于C组，P均＜0．01。结论 HuVECs分泌物可在一定程度上促进细胞迁移、抑制细胞侵袭。
목적：관찰인제정맥내피세포（ HuVECs ）분비물대자신천이화침습적영향。방법장전대적HuVECs분위3조，A、B조분별가입일정농도적FBS급HuVECs배양24、48 h적상청액，C조가일정농도적FBS급순DMEM배양액。채용세포화흔실험관찰세포천이거리，채용 Transwell 천이화침습실험관찰세포천이화침습개수。결과 A、B、C조천이거리분별위（219．27±28．44）、（163．64±19．67）、（158．18±16．36）μm；A조여B、C조비교， P균＜0．05；B、C조비교，P＞0．05。 A조천이세포수다우B、C조（P균＜0．01），B、C조천이세포수무명현차이（P＞0．05）；A조침습세포수소우B조，B조소우C조，P균＜0．01。결론 HuVECs분비물가재일정정도상촉진세포천이、억제세포침습。
Objective To observe the influence of the secretion of human umbilical vein endothelial cells (HuVECs) on their own migration and invasion .Methods The passage HuVECs were divided into 3 groups: groups A, B and C. Groups A and B were added with a certain concentration of FBS and HuVECs supernatant with 24-hour and 48-hour cultiva-tion, meanwhile, group C was added with a certain concentration of FBS and pure DMEM culture solution .Cell scratch as-say was used to observe cell migration distance .The number of cell migration and invasion of HUVECs was observed with Transwell.Results The migration distance of groups A, B and C was (219.27 ±28.44), (163.64 ±19.67) and (158.18 ±16.36) μm, respectively.Significant difference was found between group A and groups B and C ( all P<0.05);and no significant difference was found between group A and group B (P>0.05).The migrated cells in the group A were more than those of groups B and C (all P<0.01), and no significant difference in the migrated cells was found be-tween group B and group C (all P>0.05).The invasive cells of group A were less than those of group B , and the invasive cells of group B were less than those of group C (all P<0.01).Conclusion The secretion of HUVECs may promote the cell migration and inhibit the cell invasion to a certain extent .