CAJ | 학술논문

目的：探讨冠状动脉内干细胞灌注对载脂蛋白E基因缺陷小鼠离体心脏血液动力学的影响。方法设计Langendorff离体心脏灌注模型，比较接受低剂量(1.0×106)、高剂量(2.5×106)干细胞灌注时及灌注后5 min、15 min、30 min两组载脂蛋白E基因缺陷小鼠(低剂量组、高剂量组)和两组WT小鼠心脏(低剂量对照组、高剂量对照组)的血流动力学指标。同时比较注射后5 min、15 min、30 min时，两组心脏细胞的血流动力学指标。结果四组T1~T4时刻的心率均显著低于T0时刻差异有统计学意义(P<0.05)；且高剂量组的心率变化最明显。四组在进行细胞灌注后，LVEDP水平均显著升高，+dp/dt和-dp/dt水平均显著降低，且均以高剂量组的上升或下降幅度最为显著。细胞灌注后，四组的冠脉流量均较灌注前显著降低差异有统计学意义(P<0.05)；且以高剂量组的降低幅度最明显，与其他三组比较差异有统计学意义(P<0.05)。灌注后30 min，四组的漏出液细胞含量均达到了60%以上。结论冠脉内干细胞灌注会对载脂蛋白E基因缺陷小鼠离体心脏的血流动力学产生不良影响，并且灌注剂量越大，不良影响越显著。
목적：탐토관상동맥내간세포관주대재지단백E기인결함소서리체심장혈액동역학적영향。방법설계Langendorff리체심장관주모형，비교접수저제량(1.0×106)、고제량(2.5×106)간세포관주시급관주후5 min、15 min、30 min량조재지단백E기인결함소서(저제량조、고제량조)화량조WT소서심장(저제량대조조、고제량대조조)적혈류동역학지표。동시비교주사후5 min、15 min、30 min시，량조심장세포적혈류동역학지표。결과사조T1~T4시각적심솔균현저저우T0시각차이유통계학의의(P<0.05)；차고제량조적심솔변화최명현。사조재진행세포관주후，LVEDP수평균현저승고，+dp/dt화-dp/dt수평균현저강저，차균이고제량조적상승혹하강폭도최위현저。세포관주후，사조적관맥류량균교관주전현저강저차이유통계학의의(P<0.05)；차이고제량조적강저폭도최명현，여기타삼조비교차이유통계학의의(P<0.05)。관주후30 min，사조적루출액세포함량균체도료60%이상。결론관맥내간세포관주회대재지단백E기인결함소서리체심장적혈류동역학산생불량영향，병차관주제량월대，불량영향월현저。
Objective To investigate the effect of intracoronary stem cell infusion on the isolated heart hemodynamics in mice with apolipoprotein E gene defect. Methods Langendorff isolated heart perfusion model was set up. The mice with apolipoprotein E gene defect were equally divided into low-dose group [stem cells perfusion (1.0í106)] and high-dose group [stem cells perfusion (2.5í106)], and the same amount of WT mice were equally divided into low-dose control group[stem cells perfusion (1.0í106)] and high-dose control group[stem cells perfusion(2.5í106)]. The heart hemodynamics was compared between the groups at the time of perfusion, 5min, 15min, and 30min after perfusion. And the heart cells hemodynamics were compared between the groups 5min, 15min, and 30min after injection. Results The heart rate of the four groups was much lower at T1~T4 than at T0 (P<0.05);and the heart rate of high-dose group changed most significantly. After perfusion of stem cells, the levels of LVEDP increased obviously while levels of +dp/dt and -dp/dt decreased significantly in the four groups, and the high-dose group had the biggest increase or decline. Compared with before perfusion of stem cells, after perfusion, the coronary blood flow in the four groups decreased signifi-cantly,P<0.05, and the high-dose group had the biggest decline compared with the other three groups (P<0.05). 30min after perfu-sion, the proportion of cells in transudate reached more than 60%in all the four groups. Conclusion The intracoronary infusion of stem cells adversely affected the heart hemodynamics in mice with apolipoprotein E gene defect, and the greater the infusion dose was, the more significant adverse effects were.