CAJ | 학술논문

为扩大甘蓝型油菜保持系与恢复系间的遗传差异，以波里马细胞质雄性不育系的白菜型亲本保持系Ageva、甘蓝型亲本保持系105B 及相应不育系105A、8个新型甘蓝型保持系 B1～ B8（105B × Ageva 的种间杂种回交后代）及相应不育系 A1～ A8、4个甘蓝型恢复系 R1～ R4为材料，利用分子标记分析保持系与恢复系材料间的遗传差异，用 R1～ R4与甘蓝型保持系进行 NCⅡ双列杂交，测定其杂种表现，并对甘蓝型保持系相应不育系进行花瓣张开度和结实性调查。结果表明：保持系 B1、B2、B3、B4、B5和 B8与恢复系 R1～ R4的遗传距离均大于105B 与这些恢复系的遗传距离；新型甘蓝型保持系与恢复系所配组合中，有22个组合（占总组合数的68.8%）的单株产量均高于甘蓝型亲本保持系105B 与恢复系 R1～ R4所配杂交组合的单株产量。新型不育系 A1、A3的花瓣张开度和结实性与105A 接近，存在花瓣张开度大、结实性较好的新不育系材料。结论认为，导入白菜型油菜遗传成分的新甘蓝型油菜保持系应用潜力大，表现在保持系和恢复系的遗传差异大，所配组合产量高，所对应的不育系结实性好。
위확대감람형유채보지계여회복계간적유전차이，이파리마세포질웅성불육계적백채형친본보지계Ageva、감람형친본보지계105B 급상응불육계105A、8개신형감람형보지계 B1～ B8（105B × Ageva 적충간잡충회교후대）급상응불육계 A1～ A8、4개감람형회복계 R1～ R4위재료，이용분자표기분석보지계여회복계재료간적유전차이，용 R1～ R4여감람형보지계진행 NCⅡ쌍렬잡교，측정기잡충표현，병대감람형보지계상응불육계진행화판장개도화결실성조사。결과표명：보지계 B1、B2、B3、B4、B5화 B8여회복계 R1～ R4적유전거리균대우105B 여저사회복계적유전거리；신형감람형보지계여회복계소배조합중，유22개조합（점총조합수적68.8%）적단주산량균고우감람형친본보지계105B 여회복계 R1～ R4소배잡교조합적단주산량。신형불육계 A1、A3적화판장개도화결실성여105A 접근，존재화판장개도대、결실성교호적신불육계재료。결론인위，도입백채형유채유전성분적신감람형유채보지계응용잠력대，표현재보지계화회복계적유전차이대，소배조합산량고，소대응적불육계결실성호。
To broaden the variability of Brassica napus,8 new maintainer lines of B. napus were developed from interspecies hybrids of Ageva(Brassica rapa,maintainer line)and 105B(B. napus,maintainer line). Ge-netic differences among 8 new maintainer lines B1 - B8(with corresponding sterile lines A1 - A8),105B and 4 re-storer lines R1 - R4(B. napus)were estimated by SSR and AFLP techniques. To understand the hybrid perform-ance and combining ability,NCⅡdiallel crosses were implemented between R1 - R4 and B1 - B8,as well as 105B. Results showed that genetic distances between 6(B1,B2,B3,B4,B5 and B8)new maintainer lines and 4 restorer lines(R1 - R4)were larger than those between 105B and the corresponding restorer lines(R1 - R4). A-mong 32 combinations between B1 - B8 and R1 - R4,22 had higher yield per plant than those between 105B and R1 - R4. A1 and A3 had larger petal - opening angles and higher seed - bearing index for making hybrids,which were much closer to 105A. In summary,heterosis of new B. napus maintainer lines had high yield potential inclu-ding broad genetic differences,higher heterosis and plant yield.