CAJ | 학술논문

对不同环境条件下大豆脂肪酸主要组分进行 QTL 定位，为大豆脂肪酸分子标记辅助育种提供理论依据。以中黄13×东山69回交导入系群体 BC2 F2的100个家系为材料，分析回交群体的 SSR 标记多态性，结合气相色谱技术测定脂肪酸主要组分含量。构建了一张包含130个 SSR 标记的大豆遗传连锁图谱，图谱总长2433.29cM，包含19个连锁群，标记间平均遗传距离为18.86cM。共检测到与5种脂肪酸含量相关的 QTL 47个，其中有21个QTL 被重复检测到；QTL qFA - C2-4、qFA - D1b -1、qSA - J -1和 qFA - O -1连续3年均被检测到。其中 QTL qFA - C2-4检测到与亚麻酸相关，QTL qFA - D1b -1和 qSA - J -1均与硬脂酸相关，而 QTL qFA - O -1同时检测到与油酸和亚油酸相关。QTL qFA - C2-4、qFA - D1b -1、qSA - J -1和 qFA - O -1是本研究中新发现的 QTL。此外，QTL qFA - D1a -1和 qFA - C2-4是定位到的与多种脂肪酸相关的新 QTL。
대불동배경조건하대두지방산주요조분진행 QTL 정위，위대두지방산분자표기보조육충제공이론의거。이중황13×동산69회교도입계군체 BC2 F2적100개가계위재료，분석회교군체적 SSR 표기다태성，결합기상색보기술측정지방산주요조분함량。구건료일장포함130개 SSR 표기적대두유전련쇄도보，도보총장2433.29cM，포함19개련쇄군，표기간평균유전거리위18.86cM。공검측도여5충지방산함량상관적 QTL 47개，기중유21개QTL 피중복검측도；QTL qFA - C2-4、qFA - D1b -1、qSA - J -1화 qFA - O -1련속3년균피검측도。기중 QTL qFA - C2-4검측도여아마산상관，QTL qFA - D1b -1화 qSA - J -1균여경지산상관，이 QTL qFA - O -1동시검측도여유산화아유산상관。QTL qFA - C2-4、qFA - D1b -1、qSA - J -1화 qFA - O -1시본연구중신발현적 QTL。차외，QTL qFA - D1a -1화 qFA - C2-4시정위도적여다충지방산상관적신 QTL。
For molecular marker assistant breeding on higher soybean fatty acid,quantitative trait loci(QTL) mapping of predominant fatty acid components in seeds were carried on 100 BC2 lines of backcross introgression lines from Zhonghuang 13 and Dongshan 69. Gas chromatography was used to determine fatty acid components. Re-sults included a linkage map constructed with 130 polymorphism SSR markers. The total distance of the map was 2 433. 29 cM containing 19 linkage groups,and the average distance between markers was 18. 86 cM. Total 47 QTLs related to 5 fatty acid components were detected,and 21 of them were repeatable QTLs. QTL qFA - C2 - 4, qFA - J - 1,qFA - D1b - 1 and qFA - O - 1 appeared consistently over the 3 year period. Among them,qFA - C2- 4 was mapped for linolenic,qSA - J - 1 and qFA - D1b - 1 were for stearic,qFA - O - 1 were for both oleic and linolenic. QTL qFA - D1a - 1 and qFA - C2 - 4 were novel QTLs for several fatty acids. QTL qFA - C2 - 3,qFA- J - 1,qFA - D1b - 1 and qFA - O - 1 were newly reported.