CAJ | 학술논문

目的：本研究探讨整合素β1对牙龈卟啉单胞菌诱导牙龈上皮细胞MMP-2和MMP-9表达的影响。方法采用 Western blot 和 qRT-PCR 检测牙龈卟啉单胞菌感染前后牙龈上皮细胞MMP-2和MMP-9的表达；通过integrinβ1-siRNA下调牙龈上皮细胞整合素β1的表达，采用Western blot 和 qRT-PCR 检测牙龈卟啉单胞菌感染对照组和整合素β1干扰组的牙龈上皮细胞后 MMP-2和MMP-9表达。结果牙龈卟啉单胞菌感染牙龈上皮细胞可以诱导牙龈上皮细胞 MMP-2和 MMP-9的表达增加，并且表达都是在12 h时达到最高峰。下调牙龈上皮细胞整合素β1后，牙龈卟啉单胞菌诱导siRNA-integrinβ1干扰组牙龈上皮细胞MMP-2和MMP-9表达明显减少，与对照组相比存在明显差异（P＜0.01）。结论整合素β1在牙龈卟啉单胞菌诱导牙龈上皮细胞MMP-2和MMP-9表达中起很重要的作用，可能成为治疗牙周炎的新靶点。
목적：본연구탐토정합소β1대아간계람단포균유도아간상피세포MMP-2화MMP-9표체적영향。방법채용 Western blot 화 qRT-PCR 검측아간계람단포균감염전후아간상피세포MMP-2화MMP-9적표체；통과integrinβ1-siRNA하조아간상피세포정합소β1적표체，채용Western blot 화 qRT-PCR 검측아간계람단포균감염대조조화정합소β1간우조적아간상피세포후 MMP-2화MMP-9표체。결과아간계람단포균감염아간상피세포가이유도아간상피세포 MMP-2화 MMP-9적표체증가，병차표체도시재12 h시체도최고봉。하조아간상피세포정합소β1후，아간계람단포균유도siRNA-integrinβ1간우조아간상피세포MMP-2화MMP-9표체명현감소，여대조조상비존재명현차이（P＜0.01）。결론정합소β1재아간계람단포균유도아간상피세포MMP-2화MMP-9표체중기흔중요적작용，가능성위치료아주염적신파점。
Objective This study explores integrinβ1 in the role of MMP-2 and MMP-2 expression induced by porphyromonas gingivalis in gingival epithelial cell. Methods MMP-2 and MMP-9 expression was measured by Western blot and qRT-PCR in gingival epithelial cell before and after porphyromonas gingivalis infection. Next, integrinβ1 was downregulated in gingival epithelial cell by integrinβ1-siRNA, Western blot and qRT-PCR was used to determine the MMP-2 and MMP-9 expression in control and integrinβ1-siRNA group infected with porphyromonas gingivalis. Results MMP-2 and MMP-9 expression was increased after porphyromonas gingivalis infection, both at peak in 12 h. MMP-2 and MMP-9 expression in integrinβ1-siRNA group after porphyromonas gingivalis infection was decreased significantly compared with control (P<0.01, respectively). Conclusion MMP-2 and MMP-9 expression in gingival epithelial cell after porphyromonas gingivalis infection can be mediated by integirnβ1 and this may be new target to treat periodontitis.