CAJ | 학술논문

目的：对时间分辨荧光免疫法定量检测乙肝病毒血清标志物的临床意义进行观察分析。方法整群选取2012年8月-2014年8月于该院进行救治的188例乙肝病毒患者，用酶联免疫吸附试验(ELISA)和时间分辨荧光免疫分析对患者的血清标本进行乙肝标志物的定性、定量检测和比较，并且探讨其结果。结果在HBsAg和HBeAg的检出的敏感度上，两种方法差异无统计学意义(P>0.05)，TRFIA方法在抗-HBs、抗-HBe，抗-HBc的检出的敏感度明显比ELIASA法高，差异有统计学意义(P<0.05)；两种方法在1，3，5(+)、1，5(+)、1，4，5(+)及其他模式中检出率差异无统计学意义(P>0.05)；TRFIA在2(+)和2，4，5(+)检测阳性率比ELSIA差异有统计学意义(P<0.05)，全阴性模式中TRFIA阳性率显著低于ELSIA(P<0.05)。结论TRFIA法测定乙肝病毒血清标志物的相对灵敏度高，而且能够定量，值得临床推广应用。
목적：대시간분변형광면역법정량검측을간병독혈청표지물적림상의의진행관찰분석。방법정군선취2012년8월-2014년8월우해원진행구치적188례을간병독환자，용매련면역흡부시험(ELISA)화시간분변형광면역분석대환자적혈청표본진행을간표지물적정성、정량검측화비교，병차탐토기결과。결과재HBsAg화HBeAg적검출적민감도상，량충방법차이무통계학의의(P>0.05)，TRFIA방법재항-HBs、항-HBe，항-HBc적검출적민감도명현비ELIASA법고，차이유통계학의의(P<0.05)；량충방법재1，3，5(+)、1，5(+)、1，4，5(+)급기타모식중검출솔차이무통계학의의(P>0.05)；TRFIA재2(+)화2，4，5(+)검측양성솔비ELSIA차이유통계학의의(P<0.05)，전음성모식중TRFIA양성솔현저저우ELSIA(P<0.05)。결론TRFIA법측정을간병독혈청표지물적상대령민도고，이차능구정량，치득림상추엄응용。
Objective To investigate the application of the time-resolved fluorescence immunoassay in the quantitative detection of HBV M (hepatitis b virus serological markers). Methods 188 in-patients with hepatitis treated in our hospital from August 2012 to August 2014 were selected. Two kinds of methods, TRFIA (time-resolved fluorescence immunoassay) and ELISA (enzyme-linked immunosorbent assay) were used for the qualitative and quantitative detections and comparison of HBV M, and the results were discussed. Results There was no remarkable significance between ELISA and TRFIA in detecting HBsAg and HBeAg(P>0.05);but TRFIA method for the determinations of anti-HBs、anti-HBe and anti-HBc had high sensitivity (P<0.05);no obvious differences were found between ELISA and TRFIA in detecting 1, 3, 5(+) and 1, 5(+) and 1, 4, 5(+)and other modes(P>0.05);the positive de-tecting rate of TRFIA in 2(+) and 2, 4, 5(+) modes was better (P<0.05); the positive detecting rate of TRFIA in all negative mode was less (P<0.05). Conclusion Time-resolved fluorescence immunoassay for detection of HBV M has good sensitivity and good specificity and it is helpful to estimate the effect.