中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
3期
333-335
,共3页
宋潇%张婷%邵翠杰%张励才
宋瀟%張婷%邵翠傑%張勵纔
송소%장정%소취걸%장려재
氢%疼痛%炎症
氫%疼痛%炎癥
경%동통%염증
Hydrogen%Pain%Inflammation
目的 评价富氢液对大鼠慢性炎性痛的影响.方法 健康雄性SD大鼠32只,8～10周龄,体重200～250 g,采用随机数字表法,将其分为4组(n=8):对照组(C组)、完全弗氏佐剂组(CFA组)、富氢液组(H2组)和完全弗氏佐剂+富氢液组(CFA+H2组).CFA组和CFA+H2组采用左后肢足底注射完全弗氏佐剂100μl的方法制备大鼠慢性炎性痛模型.H2组和CFA+H2组于造模后1d开始腹腔注射0.6 mmol/L富氢液5 ml/kg,1次/d,连续7d;其余2组腹腔注射等容量生理盐水.分别于造模前1d和造模后1、3、7d时,测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL);于造模后7d痛阈测定结束后处死大鼠,取L4-5节段脊髓组织,采用Western blot检测核因子E2相关因子2(Nrf2)和血红素氧合酶-1(HO-1)的表达.结果 与C组比较,H2组MWT、TWL和脊髓组织Nrf2和HO-1的表达差异无统计学意义(P＞0.05),CFA组和CFA+H2组造模后各时点MWT降低,TWL缩短,脊髓组织Nrf2和HO-1的表达上调(P＜0.05);与CFA组比较,CFA+H2组造模后各时点MWT升高,TWL延长,脊髓组织Nrf2和HO-1的表达上调(P＜0.05).结论 富氢液可减轻大鼠慢性炎性痛,其机制与激活脊髓Nrf2/ARE信号通路有关.
目的 評價富氫液對大鼠慢性炎性痛的影響.方法 健康雄性SD大鼠32隻,8～10週齡,體重200～250 g,採用隨機數字錶法,將其分為4組(n=8):對照組(C組)、完全弗氏佐劑組(CFA組)、富氫液組(H2組)和完全弗氏佐劑+富氫液組(CFA+H2組).CFA組和CFA+H2組採用左後肢足底註射完全弗氏佐劑100μl的方法製備大鼠慢性炎性痛模型.H2組和CFA+H2組于造模後1d開始腹腔註射0.6 mmol/L富氫液5 ml/kg,1次/d,連續7d;其餘2組腹腔註射等容量生理鹽水.分彆于造模前1d和造模後1、3、7d時,測定機械縮足反應閾(MWT)和熱縮足潛伏期(TWL);于造模後7d痛閾測定結束後處死大鼠,取L4-5節段脊髓組織,採用Western blot檢測覈因子E2相關因子2(Nrf2)和血紅素氧閤酶-1(HO-1)的錶達.結果 與C組比較,H2組MWT、TWL和脊髓組織Nrf2和HO-1的錶達差異無統計學意義(P＞0.05),CFA組和CFA+H2組造模後各時點MWT降低,TWL縮短,脊髓組織Nrf2和HO-1的錶達上調(P＜0.05);與CFA組比較,CFA+H2組造模後各時點MWT升高,TWL延長,脊髓組織Nrf2和HO-1的錶達上調(P＜0.05).結論 富氫液可減輕大鼠慢性炎性痛,其機製與激活脊髓Nrf2/ARE信號通路有關.
목적 평개부경액대대서만성염성통적영향.방법 건강웅성SD대서32지,8～10주령,체중200～250 g,채용수궤수자표법,장기분위4조(n=8):대조조(C조)、완전불씨좌제조(CFA조)、부경액조(H2조)화완전불씨좌제+부경액조(CFA+H2조).CFA조화CFA+H2조채용좌후지족저주사완전불씨좌제100μl적방법제비대서만성염성통모형.H2조화CFA+H2조우조모후1d개시복강주사0.6 mmol/L부경액5 ml/kg,1차/d,련속7d;기여2조복강주사등용량생리염수.분별우조모전1d화조모후1、3、7d시,측정궤계축족반응역(MWT)화열축족잠복기(TWL);우조모후7d통역측정결속후처사대서,취L4-5절단척수조직,채용Western blot검측핵인자E2상관인자2(Nrf2)화혈홍소양합매-1(HO-1)적표체.결과 여C조비교,H2조MWT、TWL화척수조직Nrf2화HO-1적표체차이무통계학의의(P＞0.05),CFA조화CFA+H2조조모후각시점MWT강저,TWL축단,척수조직Nrf2화HO-1적표체상조(P＜0.05);여CFA조비교,CFA+H2조조모후각시점MWT승고,TWL연장,척수조직Nrf2화HO-1적표체상조(P＜0.05).결론 부경액가감경대서만성염성통,기궤제여격활척수Nrf2/ARE신호통로유관.
Objective To evaluate the effects of hydrogen-rich saline on chronic inflammatory pain in rats.Methods Thirty-two male Sprague-Dawley rats,aged 8-10 weeks,weighing 200-250 g,were randomly divided into 4 groups (n =8 each) using a random number table:control group (group C);complete Freund's adjuvant (CFA) group;hydrogen-rich saline group (group H2);CFA + hydrogenrich saline group (CFA+H2 group).Chronic inflammatory pain was induced by injecting CFA 100 μl into the plantar surface of the left hindpaw in CFA and CFA + H2 groups.In H2 and CFA + H2 groups,0.6 mmol/L hydrogen-rich saline 5 ml/kg was injected intraperitoneally once a day for 7 consecutive days starting from 1 day after injection of CFA,while the equal volume of normal salinc was given instead of hydrogen-rich saline in C and CFA groups.The mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured at 1 day before CFA injection and 1,3 and 7 days after CFA injection.The rats were sacrificed after the last measurement of pain threshold on day 7 after CFA injection.The left lumbar segments (L4 5) of the spinal cord were removed for determination of nuclear factor E2-related factor 2 (Nrf2) and heme oxygenase-1 (HO-1) expression by Western blot.Results Compared with C group,no significant change was found in the MWT,TWL and expression of Nrf2 and HO-1 in H2 group,and the MWT was significantly decreased,the TWL was shortened,and the expression of Nrf2 and HO-1 was up-regulated in CFA and CFA+H2 groups.Compared with CFA group,the MWT was significantly increased,the TWL was prolonged,and the expression of Nrf2 and HO-1 was up-regulated in CFA+H2 group.Conclusion Hydrogen-rich saline can alleviate chronic inflammatory pain in rats,and activation of Nrf2/ARE signaling pathway in the spinal cord is involved in the mechanism.
