中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2015年
3期
336-339
,共4页
元元%王志芬%于泳浩%王国林
元元%王誌芬%于泳浩%王國林
원원%왕지분%우영호%왕국림
受体,阿片样,δ%疼痛,手术后%哌啶类%痛觉过敏%糖原合成酶激酶3%脊髓
受體,阿片樣,δ%疼痛,手術後%哌啶類%痛覺過敏%糖原閤成酶激酶3%脊髓
수체,아편양,δ%동통,수술후%고정류%통각과민%당원합성매격매3%척수
Receptors,opioid,delta%Pain,postoperative%Piperidines%Hyperalgesia%Glycogen synthase kinase 3%Spinal cord
目的 评价瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓δ受体与糖原合成酶激酶-3β(GSK-3β)活性的关系.方法 取尾静脉置管成功的雄性SD大鼠24只,体重240～ 260 g,2～3月龄,采用随机数字表法,将其分为3组(n=8):对照组(C组)腹腔注射等容量生理盐水,静脉输注等速率生理盐水60 min;瑞芬太尼+切口痛组(R+I组)腹腔注射等容量生理盐水,静脉输注瑞芬太尼1.2μg· kg-1 ·min-1 60 min,于输注即刻制备切口痛模型;δ受体拮抗剂组(N组)腹腔注射那曲吲哚0.1mg/kg,静脉输注瑞芬太尼1.2 μg·kg-1·min-1 60 min,于输注即刻制备切口痛模型.于静脉输注生理盐水或瑞芬太尼前24 h、静脉给药后2、6、24和48 h(T04)时测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL).最后一次痛阈测定结束后处死大鼠,取脊髓L4-6节段,采用Western blot法测定GSK-3β及磷酸化GSK-3β(pGSK-3β)的表达水平,计算pGSK-3β/GSK-3β比值,采用RT-PCR法测定GSK-3βmRNA表达水平.结果 与C组比较,R+I组和N组T1-4时MWT降低,TWL缩短,脊髓组织GSK-3β、pGSK-3β和GSK-3β mRNA的表达上调,pGSK-3β/GSK-3β比值降低(P＜0.05);与R+I组比较,N组T1-4时MWT升高,TWL延长,脊髓组织GSK-3β、pGSK-3β和GSK-3β mRNA的表达下调,pGSK-3β/GSK-3β比值升高(P＜0.05).结论 瑞芬太尼诱发切口痛大鼠痛觉过敏时脊髓GSK-3β的活性增强与δ受体的激活有关.
目的 評價瑞芬太尼誘髮切口痛大鼠痛覺過敏時脊髓δ受體與糖原閤成酶激酶-3β(GSK-3β)活性的關繫.方法 取尾靜脈置管成功的雄性SD大鼠24隻,體重240～ 260 g,2～3月齡,採用隨機數字錶法,將其分為3組(n=8):對照組(C組)腹腔註射等容量生理鹽水,靜脈輸註等速率生理鹽水60 min;瑞芬太尼+切口痛組(R+I組)腹腔註射等容量生理鹽水,靜脈輸註瑞芬太尼1.2μg· kg-1 ·min-1 60 min,于輸註即刻製備切口痛模型;δ受體拮抗劑組(N組)腹腔註射那麯吲哚0.1mg/kg,靜脈輸註瑞芬太尼1.2 μg·kg-1·min-1 60 min,于輸註即刻製備切口痛模型.于靜脈輸註生理鹽水或瑞芬太尼前24 h、靜脈給藥後2、6、24和48 h(T04)時測定機械縮足反應閾(MWT)和熱縮足潛伏期(TWL).最後一次痛閾測定結束後處死大鼠,取脊髓L4-6節段,採用Western blot法測定GSK-3β及燐痠化GSK-3β(pGSK-3β)的錶達水平,計算pGSK-3β/GSK-3β比值,採用RT-PCR法測定GSK-3βmRNA錶達水平.結果 與C組比較,R+I組和N組T1-4時MWT降低,TWL縮短,脊髓組織GSK-3β、pGSK-3β和GSK-3β mRNA的錶達上調,pGSK-3β/GSK-3β比值降低(P＜0.05);與R+I組比較,N組T1-4時MWT升高,TWL延長,脊髓組織GSK-3β、pGSK-3β和GSK-3β mRNA的錶達下調,pGSK-3β/GSK-3β比值升高(P＜0.05).結論 瑞芬太尼誘髮切口痛大鼠痛覺過敏時脊髓GSK-3β的活性增彊與δ受體的激活有關.
목적 평개서분태니유발절구통대서통각과민시척수δ수체여당원합성매격매-3β(GSK-3β)활성적관계.방법 취미정맥치관성공적웅성SD대서24지,체중240～ 260 g,2～3월령,채용수궤수자표법,장기분위3조(n=8):대조조(C조)복강주사등용량생리염수,정맥수주등속솔생리염수60 min;서분태니+절구통조(R+I조)복강주사등용량생리염수,정맥수주서분태니1.2μg· kg-1 ·min-1 60 min,우수주즉각제비절구통모형;δ수체길항제조(N조)복강주사나곡신타0.1mg/kg,정맥수주서분태니1.2 μg·kg-1·min-1 60 min,우수주즉각제비절구통모형.우정맥수주생리염수혹서분태니전24 h、정맥급약후2、6、24화48 h(T04)시측정궤계축족반응역(MWT)화열축족잠복기(TWL).최후일차통역측정결속후처사대서,취척수L4-6절단,채용Western blot법측정GSK-3β급린산화GSK-3β(pGSK-3β)적표체수평,계산pGSK-3β/GSK-3β비치,채용RT-PCR법측정GSK-3βmRNA표체수평.결과 여C조비교,R+I조화N조T1-4시MWT강저,TWL축단,척수조직GSK-3β、pGSK-3β화GSK-3β mRNA적표체상조,pGSK-3β/GSK-3β비치강저(P＜0.05);여R+I조비교,N조T1-4시MWT승고,TWL연장,척수조직GSK-3β、pGSK-3β화GSK-3β mRNA적표체하조,pGSK-3β/GSK-3β비치승고(P＜0.05).결론 서분태니유발절구통대서통각과민시척수GSK-3β적활성증강여δ수체적격활유관.
Objective To evaluate the relationship between delta opioid receptors (DORs) and activity of glycogen synthase kinase-3β (GSK-3β) in the spinal cord during hyperalgesia induced by remifentanil in a rat model of incisional pain (IP).Methods Twenty-four male Sprague-Dawley rats,aged 240-260 g,weighing 2-3 months,were randomly divided into 3 groups (n =8 each) using a random number table:control group (group C),remifentanil+IP group (group R+I) and DOR antagonist naltrindole group (group N).A 1-cm longitudinal incision was made in the plantar surface of the left hindpaw in anesthetized rats.In group C,the equal volume of normal saline was injected intraperitoneally,and normal saline was then infused for 60 min at the same rate.In group R+I,the equal volume of normal saline was injected intraperitoneally,remifentanil was infused for 60 min at 1.2 μg · kg-1 · min-1,and IP was produced immediately after onset of remifentanil infusion.In group N,naltrindole 0.1 mg/kg was injected intraperitoneally,remifentanil was infused for 60 min at 1.2 μg · kg-1 · min-1,and IP was produced immediately after onset of remifentanil infusion.At 24 h before infusion of normal saline or remifentanil and 2,6,24 and 48 h after iv administration (T0-4),the mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured.The rats were sacrificed after the last measurement of pain threshold,the lumbar segment (L4-6) of the spinal cord was removed for determination of the expression of GSK-3β,phosphor-GSK-3β (pGSK-3β) (by Western blot) and GSK-3β mRNA (realtime PCR).The ratio of pGSK-3β /GSK-3β was calculated.Results Compared with group C,the MWT was significantly decreased,and the TWL was shortened at T1 4,the expression of GSK-3β,pGSK-3β and GSK-3β mRNA was up-regulated,and pGSK-3β/GSK-3β ratio was decreased in R + I and N groups.Compared with group R + I,thc MWT was significantly incrcased,and the TWL was prolonged at T1-4,the expression of GSK-3β,pGSK-3β and GSK-3β rmRNA was down-regulated,and pGSK-3β/GSK-3β ratio was increased in group N.Conclusion Activation of DOR is involved in enhancement of activity of GSK-3β in the spinal cord during hyperalgesia induced by remifentanil in a rat model of IP.
