CAJ | 학술논문

目的评价脊髓多巴胺D2受体在大鼠神经病理性痛中的作用.方法健康雄性SD大鼠30只,6～8周龄,体重180～ 200 g,采用随机数字表法,将其分为5组(n=6):正常对照组(C组)、假手术组(S组)、神经病理性痛组(NP组)、生理盐水组(N组)和多巴胺D2受体激动剂喹吡罗组(Q组).采用坐骨神经慢性压迫性损伤法建立大鼠神经病理性痛模型.于术后7d时,N组经30s鞘内注射生理盐水10 μl,Q组经30 s鞘内注射多巴胺D2受体激动剂喹吡罗10μg(溶于10 μl生理盐水中).于术前1d、术后3和7d、给药后30 min、1、2、4、8和16 h(T08)时,测定机械缩足反应阈(MWT)和热缩足潜伏期(TWL).结果 C组和S组各时点MWT和TWL比较差异无统计学意义(P＞0.05);与C组和S组比较,NP组、N组和Q组T1-8时MWT降低,TWL缩短(P＜0.05);与NP组比较,N组各时点各指标差异无统计学意义(P＞0.05),Q组T4-6时MWT升高,TWL延长(P＜0.05).结论脊髓多巴胺D2受体功能抑制参与了大鼠神经病理性痛的维持.
목적 평개척수다파알D2수체재대서신경병이성통중적작용.방법 건강웅성SD대서30지,6～8주령,체중180～ 200 g,채용수궤수자표법,장기분위5조(n=6):정상대조조(C조)、가수술조(S조)、신경병이성통조(NP조)、생리염수조(N조)화다파알D2수체격동제규필라조(Q조).채용좌골신경만성압박성손상법건립대서신경병이성통모형.우술후7d시,N조경30s초내주사생리염수10 μl,Q조경30 s초내주사다파알D2수체격동제규필라10μg(용우10 μl생리염수중).우술전1d、술후3화7d、급약후30 min、1、2、4、8화16 h(T08)시,측정궤계축족반응역(MWT)화열축족잠복기(TWL).결과 C조화S조각시점MWT화TWL비교차이무통계학의의(P＞0.05);여C조화S조비교,NP조、N조화Q조T1-8시MWT강저,TWL축단(P＜0.05);여NP조비교,N조각시점각지표차이무통계학의의(P＞0.05),Q조T4-6시MWT승고,TWL연장(P＜0.05).결론 척수다파알D2수체공능억제삼여료대서신경병이성통적유지.
Objective To evaluate the role of spinal dopamine D2 receptors in a rat model of neuropathic pain.Methods Thirty healthy male Sprague-Dawley rats,aged 6-8 weeks,weighing 180-200 g,wcre randomly divided into 5 groups (n =6 each) using a random number table:control group (group C),sham operation group (group S),neuropathic pain group (group NP),normal saline group (group N) and dopamine D2 receptor agonist quinpirole group (group Q).Neuropathic pain was produced by chronic constriction injury of the sciatic nerve (CCI) in rats anesthetized with intraperitoneal 2％ pentobarbital sodium 40 mg/kg.At 7 days after CCI,normal saline 10 μl was injected intrathecally over 30 s in group N,and quinpirole 10 μg (in 10 μl of normal saline) was injected intrathecally over 30 s in group Q.At 1 day before CCI,3 and 7 days aher CCI,and 30 min and 1,2,4,8 and 16 h after administration,mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) were measured.Results There was no significant difference in MWT and TWL at each time point between group C and group S.MWT was significantly lower,and TWL was shorter at T1-8 in NP,N and Q groups than in C and S groups.Compared with group N,no significant change was found in MWT and TWL at each time point in N group,and MWT was significantly increased,and TWL was prolonged at T4-6 in group Q.Conclusion Inhibited function of spinal dopamine D2 receptors is involved in the maintenance of neuropathic pain in rats.