CAJ | 학술논문

目的研究高迁移率族蛋白B1 (HMGB 1)/Toll样受体4(TLR4)在胶质瘤瘤体及瘤周组织的表达情况,探讨胶质瘤相关性癫痫的发生机制. 方法收集郑州大学第五附属医院神经外科自2011年6月至2014年6月手术切除的95例胶质瘤标本,其中低级别胶质瘤(WHO Ⅰ～Ⅱ级)55例,高级别胶质瘤(WHOⅢ～Ⅳ)级40例.免疫组化染色、RT-PCR分别检测胶质瘤瘤体、瘤周组织HMGB1及TLR4蛋白和mRNA的表达. 结果 95例胶质瘤患者中,伴癫痫发作34例(35.79％).低级别胶质瘤患者的癫痫发生率(49.09％)高于高级别胶质瘤患者(17.50％),差异有统计学意义(x2=10.057,P=0.002);伴发癫痫胶质瘤瘤周组织中HMGB1、TLR4蛋白表达阳性率和mRNA表达均高于不伴癫痫胶质瘤瘤周组织,差异有统计学意义(P＜0.05);伴、不伴癫痫胶质瘤瘤体组织HMGB1、TLR4蛋白和mRNA的表达比较差异均无统计学意义(P＞0.05). 结论瘤周组织中高表达的HMGB1及TLR4可能参与胶质瘤相关性癫痫的发生发展,有可能成为胶质瘤相关性癫痫治疗的新靶点.
목적 연구고천이솔족단백B1 (HMGB 1)/Toll양수체4(TLR4)재효질류류체급류주조직적표체정황,탐토효질류상관성전간적발생궤제. 방법 수집정주대학제오부속의원신경외과자2011년6월지2014년6월수술절제적95례효질류표본,기중저급별효질류(WHO Ⅰ～Ⅱ급)55례,고급별효질류(WHOⅢ～Ⅳ)급40례.면역조화염색、RT-PCR분별검측효질류류체、류주조직HMGB1급TLR4단백화mRNA적표체. 결과 95례효질류환자중,반전간발작34례(35.79％).저급별효질류환자적전간발생솔(49.09％)고우고급별효질류환자(17.50％),차이유통계학의의(x2=10.057,P=0.002);반발전간효질류류주조직중HMGB1、TLR4단백표체양성솔화mRNA표체균고우불반전간효질류류주조직,차이유통계학의의(P＜0.05);반、불반전간효질류류체조직HMGB1、TLR4단백화mRNA적표체비교차이균무통계학의의(P＞0.05). 결론 류주조직중고표체적HMGB1급TLR4가능삼여효질류상관성전간적발생발전,유가능성위효질류상관성전간치료적신파점.
Objective To investigate the expression of high mobility group protein b 1 (HMGPB1) and toll-like receptors 4 (TLR4) in human glioma tissues of patients with epilepsy and explore the mechanism of glioma-associated epilepsy.Methods Ninety-five glioma specimens,collected during the resection surgery in our hospital firom June 2011 to June 2014,were used in our experiment;among them,55 were low-grade gliomas and 40 were high-grade gliomas.Immunohistochemistry and real time-PCR were used to assay the protein and mRNA expressions of HMGB1 and TLR4 in glioma tissues and peritumoral tissues,and the relation between HMGB1/TLR4 and glioma-associated epilepsy was analyzed.Results The incidence of glioma-associated epilepsy was 35.79％ (34/95),and the incidence of glioma-associated epilepsy in low-grade gliomas (49.09％) was significantly higher than that in high-grade gliomas (17.50％)(x2=10.057,P=0.002).Immunohistochemisty and RT-PCR showed that the protein and mRNA expressions of HMGB1/TLR4 in the peritumoral tissues with glioma-associated epilepsy were significantly higher than those in the peritumoral tissues without glioma-associated epilepsy (P＜0.05),while no significant difference was noted between glioma tissues with and without epilepsy (P＞0.05).Conclusion Over-expression of HMGB1 and TLR4 in peritumoral tissues of glioma patients may be related with the development of glioma-associated epilepsy,which may be a new target in glioma therapy.