CAJ | 학술논문

目的探讨外周全血miR-200c-3p、miR-15b-5p、miR-99b-5p和miR-150-5p表达水平与缺血性脑卒中(IS)发生的关联性,以及其作为IS生物标记物的可行性. 方法选择45例2013年7月至11月在深圳市人民医院确诊的IS初发病例,以及45例与之年龄性别相匹配的在同地区社区健康服务中心做常规健康体检的对照,采用实时定量PCR检测外周全血miR-200c-3p、miR-15b-5p、miR-99b-5p和miR-150-5p的表达水平,多元Logistic回归分析miRNA表达水平与缺IS发生的关联,受试者工作特征曲线(ROC)分析miRNA是否可作为判断IS的依据. 结果 IS病例外周血miR-200c-3p和miR-15b-5p表达水平显著高于对照,而miR-99b-5p和miR-150-5p表达水平显著低于对照者,差异均有统计学意义(P＜0.05).miR-200c-3p、miR-99b-5p和miR-150-5p与IS发生具有显著关联[OR值和95％CI分别为6.30 (1.46～27.20)、0.10 (0.02～0.43)和0.06(0.01～0.30);P值分别为0.014、0.002、0.001].与传统危险因素模型相比,加入单个或2个以上miRNA都可以显著增加曲线下面积且差异有统计学意义(P＜0.05). 结论 miR-200c-3p、miR-99b-5p和miR-150-5p的表达水平与IS发生具有显著关联,有望作为IS的生物标记物.
목적 탐토외주전혈miR-200c-3p、miR-15b-5p、miR-99b-5p화miR-150-5p표체수평여결혈성뇌졸중(IS)발생적관련성,이급기작위IS생물표기물적가행성. 방법 선택45례2013년7월지11월재심수시인민의원학진적IS초발병례,이급45례여지년령성별상필배적재동지구사구건강복무중심주상규건강체검적대조,채용실시정량PCR검측외주전혈miR-200c-3p、miR-15b-5p、miR-99b-5p화miR-150-5p적표체수평,다원Logistic회귀분석miRNA표체수평여결IS발생적관련,수시자공작특정곡선(ROC)분석miRNA시부가작위판단IS적의거. 결과 IS병예외주혈miR-200c-3p화miR-15b-5p표체수평현저고우대조,이miR-99b-5p화miR-150-5p표체수평현저저우대조자,차이균유통계학의의(P＜0.05).miR-200c-3p、miR-99b-5p화miR-150-5p여IS발생구유현저관련[OR치화95％CI분별위6.30 (1.46～27.20)、0.10 (0.02～0.43)화0.06(0.01～0.30);P치분별위0.014、0.002、0.001].여전통위험인소모형상비,가입단개혹2개이상miRNA도가이현저증가곡선하면적차차이유통계학의의(P＜0.05). 결론 miR-200c-3p、miR-99b-5p화miR-150-5p적표체수평여IS발생구유현저관련,유망작위IS적생물표기물.
Objective To investigate the association between expression levels of peripheral blood miR-200c-3p,miR-15b-5p,miR-99b-5p and miR-150-5p and occurrence of ischemic stroke (IS),and to explore if these miRNAs can be biomarkers for IS.Methods Forty-five newly-diagnosed IS patients enrolled in our hospital from July to November 2013 and 45 age-and gender-matched controls from physical examination in the community health center were chosen in our study;the expression levels of whole blood miR-200c-3p,miR-15b-5p,miR-99b-5p and miR-150-5p were detected by real-time quantitative-PCR.Multivariate Logistic regression analysis was applied to evaluate the associations between miRNA expression levels and occurrence of IS.The receiver operating characteristic curve (ROC) analysis was conducted to assess the performance ofmiRNAs as biomarker for IS.Results As compared with those in the controls,the expression levels ofmiR-200c-3p and miR-15b-5p in IS patients were significantly higher,while miR-99b-5p and miR-150-5p expression levels in IS patients were significantly lower (P＜0.05).The expression levels of miR-200c-3p,miR-99b-5p and miR-150-5p were significantly associated with the occurrence of IS (adjusted odds ratio [OR]:6.30,95％ confidence interval [CI]:1.46-27.20 and P=0.014;OR:0.10,95％CI:0.02-0.43 and P=0.002;OR:0.06,95％CI:0.01-0.30 and P=0.001,respectively).Results of the ROC analysis showed that addition of one miRNA or miRNA panels to traditional risk factors led to significant improvement in the area under the curve (P＜0.05).Conclusion The expression levels of miR-200c-3p,miR-99b-5p and miR-150-5p are significantly associated with the occurrence of IS,and have potential as biomarkers for IS.