CAJ | 학술논문

从南方红豆杉菌根菌中筛选出产紫杉醇和紫杉烷类化合物10-去乙酰巴卡亭Ⅲ(10-DAB)的高产菌株,对分离获得的21株菌株进行了液体发酵培养实验,通过TLC、HPLC及LC-MS检测发现,有10株菌株具有产紫杉醇及紫杉烷类化合物的能力,其中4株菌株中产10-DAB分别为260.05、92.88、393.27、70.52μg/L,但未检出产紫杉醇；6株中产紫杉醇分别为434.94、1242.19、200.65、360.77、317.90、29.82μg/L,但未检出产10-DAB,说明南方红豆杉的各种菌根真菌虽具有合成紫杉醇或紫杉烷类化合物的能力,但各自所合成化合物有差异,不同种类的菌根菌可能分别参与了紫杉醇生物合成的不同环节,即南方红豆杉菌根高含量紫杉醇的生物合成可能是多种菌根菌协同参与作用的结果,且其中的高产菌株具有发酵生产紫杉醇的潜力。
종남방홍두삼균근균중사선출산자삼순화자삼완류화합물10-거을선파잡정Ⅲ(10-DAB)적고산균주,대분리획득적21주균주진행료액체발효배양실험,통과TLC、HPLC급LC-MS검측발현,유10주균주구유산자삼순급자삼완류화합물적능력,기중4주균주중산10-DAB분별위260.05、92.88、393.27、70.52μg/L,단미검출산자삼순；6주중산자삼순분별위434.94、1242.19、200.65、360.77、317.90、29.82μg/L,단미검출산10-DAB,설명남방홍두삼적각충균근진균수구유합성자삼순혹자삼완류화합물적능력,단각자소합성화합물유차이,불동충류적균근균가능분별삼여료자삼순생물합성적불동배절,즉남방홍두삼균근고함량자삼순적생물합성가능시다충균근균협동삼여작용적결과,차기중적고산균주구유발효생산자삼순적잠력。
To screen high-yield strains which could produce paclitaxel and 10-deacetylbaccation Ⅲ(10-DAB) from T. chinensis var. mairei mycorrhizal fungus, 21 strains were separated, obtained and carried on liquid fermentation culture. The detection results of TLC,HPLC,and LC-MS show that 10 strains among those possess the ability to produce paclitaxel and taxane,and 10-DAB contents of 4 strains are 260. 05, 92. 88,393. 27 and 70. 52 μg/L,respectively. The paclitaxel contents of 6 strains are 434. 94,1 242. 19,200. 65,360. 77,317. 90 and 29. 82 μg/L among these 10 strains. These results indicate that the mycorrhizal fungus has the ability to synthesize paxlitaxel and taxanes, but each of the synthesized compounds have the difference. It is the different kinds of mycorrhizal that may participate in the taxol biosynthesis, and high contents of taxol result form collaborative actions. And there is the paclitaxel potential to produce in fermentation culture of the high-yield strains.