CAJ | 학술논문

从黄河三角洲盐碱地土壤分离到一株耐高盐菌株NM‐1．对该菌株生理生化性质、耐盐性以及系统分类位置进行了分析．结果表明：菌株NM‐1为长杆状，革兰氏阴性，菌落黄色，呈圆形，湿润、光滑．能利用葡萄糖、乳糖和蔗糖，不能利用淀粉和明胶．生长pH范围为8.0～10.0，最高耐盐浓度（NaCl）为30％，为中度嗜盐微生物，最适生长的 Na2CO3浓度为0.25 M ．16S rDNA 序列分析表明：该菌株与 Halomonas variabilis （JN645866）以及 Halomonas variabilis （AY204638）序列的相似性均为98％，结合其形态、生理生化性质，认定该菌株属于耐盐单胞菌属，暂命名为 H alomonas sp ．NM‐1（AB917466）．
종황하삼각주염감지토양분리도일주내고염균주NM‐1．대해균주생리생화성질、내염성이급계통분류위치진행료분석．결과표명：균주NM‐1위장간상，혁란씨음성，균락황색，정원형，습윤、광활．능이용포도당、유당화자당，불능이용정분화명효．생장pH범위위8.0～10.0，최고내염농도（NaCl）위30％，위중도기염미생물，최괄생장적 Na2CO3농도위0.25 M ．16S rDNA 서렬분석표명：해균주여 Halomonas variabilis （JN645866）이급 Halomonas variabilis （AY204638）서렬적상사성균위98％，결합기형태、생리생화성질，인정해균주속우내염단포균속，잠명명위 H alomonas sp ．NM‐1（AB917466）．
A high salt tolerant strain NM‐1 was isolated from Yellow River Delta saline‐alkali soil and was characterized for its morphology , biochemical properties , the grow th under different concentration of NaCl and Na2 CO3 ,and 16S rDNA sequences .The results showed that strain NM‐1 was gram negative ,rods .Colonies were yellow ,round ,moist and smooth . Strain NM‐1 could utilize glucose ,lactose and sucrose ,not starch and gelatine .The growth pH ranges was from 8.0 to 10.0 . The tolerant concentration of NaCl for strain NM‐1 reached up to 30% .The optimum concentration of Na2CO3 for strain NM‐1 to grow was 0.25 M .16S rDNA sequences analysis proved the high homology of 98% between strain NM‐1 with H alomonas v ariabilis (AY204638 ) and H alomonas v ariabilis (JN645866 ) . Based on its morphology and biochemical properties , strain NM‐1was regarded as one member of Halomonas genus and named as H alomonas sp .NM‐1(AB917466) .