中华地方病学杂志
中華地方病學雜誌
중화지방병학잡지
Chinese Journal of Endemiology
2015年
6期
416-420
,共5页
肖春霞%赵鸿雁%侯临平%荣蓉%刘熹%赵赤鸿%朴东日%赵娜%姜海
肖春霞%趙鴻雁%侯臨平%榮蓉%劉熹%趙赤鴻%樸東日%趙娜%薑海
초춘하%조홍안%후림평%영용%류희%조적홍%박동일%조나%강해
布鲁杆菌%鉴定%生化功能%全自动微生物分析仪
佈魯桿菌%鑒定%生化功能%全自動微生物分析儀
포로간균%감정%생화공능%전자동미생물분석의
Brucella%Identification%Biochemical function%Automatic microbial identification analyzer
目的 鉴定与分析布鲁杆菌的生化特征,评价对全自动微生物分析系统的鉴定效果和临床意义.方法 17株标准菌株和121株实验菌株来自于中国疾病预防控制中心传染病预防控制所布鲁杆菌病(简称布病)研究室菌种库,实验菌株为全国26个省(市、自治区)1957-2014年历次流行自布病患者和羊、黄羊、岩羊、牛、猪分离的布鲁杆菌菌株.用GN鉴定卡在VITEK2 COMPACT全自动微生物分析系统上对标准菌株和实验菌株进行生化检测,根据布鲁杆菌属和种的生化阳性鉴定标准,通过对比分析,获得布鲁杆菌属和种的生化阳性鉴定结果.对系统鉴定异常的菌种,采用传统鉴定方法进行氧化酶、脲酶、动力、硫化氢测定和碱性复红敏感性试验、噬菌体裂解试验及A/M单相特异性血清凝集试验进行菌种的重新判定.结果 对138株布鲁杆菌菌株的全自动微生物分析系统生化鉴定结果显示,布鲁杆菌属的主要阳性鉴定指标为L-脯氨酸芳胺酶(ProA)、酪氨酸芳胺酶(TyrA)、尿素酶(URE)、氨基乙酸芳胺酶(GlyA)、乳酸盐产碱(1LATK)、ELLMAN (ELLM);与系统值比较,全部菌株生化功能相似率为97.99％(135.23/138),其中标准菌株为96.71％(16.44/ 17),实验菌株为98.17％(118.79/121);菌株鉴定需要的时间为6.1 ～ 7.7 h,其中标准菌株为7.3 h,实验菌株为6.9 h.区分是否为布鲁杆菌属的阳性鉴定指标为ProA、TyrA、URE、GlyA;区分羊种布鲁杆菌的阳性指标为ELLM;区分牛种布鲁杆菌的阳性指标为1LATK;区分猪种布鲁杆菌的阳性指标为丙氨酸-苯丙氨酸-脯氨酸芳胺酶(APPA);犬种无明显的阳性指标.从实验菌株中鉴定出4株人苍白杆菌,传统方法显示,氧化酶阳性、脲酶阳性、硫化氢阳性、动力阴性;碱性复红敏感性试验有抗性2株(牛种),敏感2株(猪种);硫堇实验敏感2株(牛种),有抗性2株(猪种);噬菌体裂解试验阳性2株(牛种),阴性2株(猪种).A/M因子单相特异性血清凝集试验,牛种布鲁杆菌(A)阳性,羊种布鲁杆菌(M)阴性,排除人苍白杆菌,最终鉴定均为布鲁杆菌种.结论 全自动微生物分析系统在鉴定布鲁杆菌时速度快,可靠性较高,对不同菌株属和种鉴定的主要阳性指标明确,可用于临床诊断.当鉴定为人苍白杆菌时,需与布鲁杆菌进行鉴别,以免误诊.
目的 鑒定與分析佈魯桿菌的生化特徵,評價對全自動微生物分析繫統的鑒定效果和臨床意義.方法 17株標準菌株和121株實驗菌株來自于中國疾病預防控製中心傳染病預防控製所佈魯桿菌病(簡稱佈病)研究室菌種庫,實驗菌株為全國26箇省(市、自治區)1957-2014年歷次流行自佈病患者和羊、黃羊、巖羊、牛、豬分離的佈魯桿菌菌株.用GN鑒定卡在VITEK2 COMPACT全自動微生物分析繫統上對標準菌株和實驗菌株進行生化檢測,根據佈魯桿菌屬和種的生化暘性鑒定標準,通過對比分析,穫得佈魯桿菌屬和種的生化暘性鑒定結果.對繫統鑒定異常的菌種,採用傳統鑒定方法進行氧化酶、脲酶、動力、硫化氫測定和堿性複紅敏感性試驗、噬菌體裂解試驗及A/M單相特異性血清凝集試驗進行菌種的重新判定.結果 對138株佈魯桿菌菌株的全自動微生物分析繫統生化鑒定結果顯示,佈魯桿菌屬的主要暘性鑒定指標為L-脯氨痠芳胺酶(ProA)、酪氨痠芳胺酶(TyrA)、尿素酶(URE)、氨基乙痠芳胺酶(GlyA)、乳痠鹽產堿(1LATK)、ELLMAN (ELLM);與繫統值比較,全部菌株生化功能相似率為97.99％(135.23/138),其中標準菌株為96.71％(16.44/ 17),實驗菌株為98.17％(118.79/121);菌株鑒定需要的時間為6.1 ～ 7.7 h,其中標準菌株為7.3 h,實驗菌株為6.9 h.區分是否為佈魯桿菌屬的暘性鑒定指標為ProA、TyrA、URE、GlyA;區分羊種佈魯桿菌的暘性指標為ELLM;區分牛種佈魯桿菌的暘性指標為1LATK;區分豬種佈魯桿菌的暘性指標為丙氨痠-苯丙氨痠-脯氨痠芳胺酶(APPA);犬種無明顯的暘性指標.從實驗菌株中鑒定齣4株人蒼白桿菌,傳統方法顯示,氧化酶暘性、脲酶暘性、硫化氫暘性、動力陰性;堿性複紅敏感性試驗有抗性2株(牛種),敏感2株(豬種);硫堇實驗敏感2株(牛種),有抗性2株(豬種);噬菌體裂解試驗暘性2株(牛種),陰性2株(豬種).A/M因子單相特異性血清凝集試驗,牛種佈魯桿菌(A)暘性,羊種佈魯桿菌(M)陰性,排除人蒼白桿菌,最終鑒定均為佈魯桿菌種.結論 全自動微生物分析繫統在鑒定佈魯桿菌時速度快,可靠性較高,對不同菌株屬和種鑒定的主要暘性指標明確,可用于臨床診斷.噹鑒定為人蒼白桿菌時,需與佈魯桿菌進行鑒彆,以免誤診.
목적 감정여분석포로간균적생화특정,평개대전자동미생물분석계통적감정효과화림상의의.방법 17주표준균주화121주실험균주래자우중국질병예방공제중심전염병예방공제소포로간균병(간칭포병)연구실균충고,실험균주위전국26개성(시、자치구)1957-2014년력차류행자포병환자화양、황양、암양、우、저분리적포로간균균주.용GN감정잡재VITEK2 COMPACT전자동미생물분석계통상대표준균주화실험균주진행생화검측,근거포로간균속화충적생화양성감정표준,통과대비분석,획득포로간균속화충적생화양성감정결과.대계통감정이상적균충,채용전통감정방법진행양화매、뇨매、동력、류화경측정화감성복홍민감성시험、서균체렬해시험급A/M단상특이성혈청응집시험진행균충적중신판정.결과 대138주포로간균균주적전자동미생물분석계통생화감정결과현시,포로간균속적주요양성감정지표위L-포안산방알매(ProA)、락안산방알매(TyrA)、뇨소매(URE)、안기을산방알매(GlyA)、유산염산감(1LATK)、ELLMAN (ELLM);여계통치비교,전부균주생화공능상사솔위97.99％(135.23/138),기중표준균주위96.71％(16.44/ 17),실험균주위98.17％(118.79/121);균주감정수요적시간위6.1 ～ 7.7 h,기중표준균주위7.3 h,실험균주위6.9 h.구분시부위포로간균속적양성감정지표위ProA、TyrA、URE、GlyA;구분양충포로간균적양성지표위ELLM;구분우충포로간균적양성지표위1LATK;구분저충포로간균적양성지표위병안산-분병안산-포안산방알매(APPA);견충무명현적양성지표.종실험균주중감정출4주인창백간균,전통방법현시,양화매양성、뇨매양성、류화경양성、동력음성;감성복홍민감성시험유항성2주(우충),민감2주(저충);류근실험민감2주(우충),유항성2주(저충);서균체렬해시험양성2주(우충),음성2주(저충).A/M인자단상특이성혈청응집시험,우충포로간균(A)양성,양충포로간균(M)음성,배제인창백간균,최종감정균위포로간균충.결론 전자동미생물분석계통재감정포로간균시속도쾌,가고성교고,대불동균주속화충감정적주요양성지표명학,가용우림상진단.당감정위인창백간균시,수여포로간균진행감별,이면오진.
Objective To identify and analyse the biochemical characterization of brucella and to evaluate its clinical application by VITEK2 COMPACT automatic microbial identification analyzer.Methods Seventeen strains of standard strains and 121 strains of experimental strains were from bacteria storehouse of brucella disease,Institute of Infectious Diseases Prevention and Control,China Center for Disease Control and Prevention.Experimental strains were from 26 provinces (municipalities and autonomous regions) from 1957 to 2014,including all previous strains from patients and goats,antelope,sheep,cattle,and pig.Reference standard strains and experimental strains were analyzed using the GN identification card on VITEK2 COMPACT automatic microbial identification analyzer,and biochemical identification of brucella strains was done.Identified abnormal strains were rechecked by traditional test methods,including oxidase experiment,urease experiment,semisolid experiment,determination of hydrogen sulfide experiment,basic fuchsin susceptibility experiment,phage lysis experiment,and A/M single-phase specific serum agglutination experiment.Results Of the 138 strains of brucella analyzed by the automatic microbial identification system,the results showed that the main identification indicators of brucella genus were:L-proline arylamidase (ProA),tyrosine arylamidase (TyrA),urease (URE),glycine arylamidase (GlyA),L-lactate alkalinisation (1LATK),and ELLMAN (ELLM).Compared with the system values,all strains biochemical function similar rate was 97.99％ (135.23/138),including standard strains was 96.71％ (16.44/17),experimental strains was 98.17％ (118.79/ 121);time required for strains identification was 6.1-7.7 h,including standard strains was 7.3 h,experimental strains was 6.9 h.Identification indicators for distinguish brucella species were:ProA,TyrA,URE,and GlyA;for distinguish brucella melitensis was ELLM;for distinguish brucella abortus was 1LATK;for distinguish brucella suis was Ala-Phe-Pro-arylamidase (APPA);no obvious positive brucella indicator was found for brucella cains.From the experimental strains 4 strains of ochrobactrum anthropi were identified;the traditional methods showed that they were oxidase-positive,urase-positive,and hydrogen sulfide-positive,power-negative;basic fuchsin susceptibility test discovered 2 strains (burcella abortus),sensitive 2 strains (burcella suis);sulfur sensitive experiment 2 strains pansy (burcella abortus),resistant 2 strains (burcella suis);phage lysis test-positive 2 strains (burcella abortus),and negative 2 strains (burcella suis).A/M single-phase specific serum agglutination experiment showed that they were brucella abortus (A)-positive and brucella melitensis (M)-negative.They were not ochrobactrum anthropi.The identification results showed that they were brucella.Conclusions The VITEK2 COMPACT automatic microbial analysis system has a speed and high reliability in identification of brucella;the main positive indicators are clear for identification of different strains of genera and species;it can be used for clinical diagnosis.When the identification result is ochrobactrum anthropi,it should be identified with brucella in order to avoid misdiagnosis.
