CAJ | 학술논문

对造纸废弃物麦草废渣进行高温自水解预处理,并对预处理后的麦草废渣进行组成分析和酶解,以纤维素和木聚糖的酶解得率为考核指标,研究了不同自水解预处理条件对麦草废渣酶解的影响。研究表明,预处理后麦草废渣的化学组成和物理结构均发生了改变,纤维素的含量增加,比表面积增大且促进了纤维素的酶解。酶解结果显示,温度对预处理麦草废渣的酶水解得率有着极其显著的影响。以200℃自水解预处理60 min的麦草废渣为酶水解底物(质量浓度50 g/L),添加纤维素酶35 FPIU/g(以纤维素计,下同)、β-葡萄糖苷酶25 IU/g、木聚糖酶120 U/g及2 g/L聚乙二醇(PEG6000),酶解体系50 mL,于50℃酶解36 h,其纤维素和木聚糖酶解得率分别达86.29%和74.03%,是麦草废渣经170℃自水解40 min后酶解得率的2.3倍和2.8倍。
대조지폐기물맥초폐사진행고온자수해예처리,병대예처리후적맥초폐사진행조성분석화매해,이섬유소화목취당적매해득솔위고핵지표,연구료불동자수해예처리조건대맥초폐사매해적영향。연구표명,예처리후맥초폐사적화학조성화물리결구균발생료개변,섬유소적함량증가,비표면적증대차촉진료섬유소적매해。매해결과현시,온도대예처리맥초폐사적매수해득솔유착겁기현저적영향。이200℃자수해예처리60 min적맥초폐사위매수해저물(질량농도50 g/L),첨가섬유소매35 FPIU/g(이섬유소계,하동)、β-포도당감매25 IU/g、목취당매120 U/g급2 g/L취을이순(PEG6000),매해체계50 mL,우50℃매해36 h,기섬유소화목취당매해득솔분별체86.29%화74.03%,시맥초폐사경170℃자수해40 min후매해득솔적2.3배화2.8배。
Wheat straw from papermaking waste was pretreated by hot-compressed water. The test of enzymatic hydrolysis and its compositions was studied. The effects of pretreatment conditions on waste wheat straw were investigated, and the enzymatic hydrolysis yields of cellulose and xylan were examined. Results showed that chemical composition of waste wheat straw and physical structures were changed after pretreatment, and the cellulose contents and surfaces increased. This promoted the enzymatic hydrolysis of cellulose. Enzymatic hydrolysis results showed that the temperature of pretreatment had a very significant impact on the yield. Waste wheat straw pretreated at 200 ℃ for 60min was used as the enzyme hydrolysis substrate at a concentration of 50 g/L. Under the conditions of cellulose enzyme 35 FPIU/g ( of cellulose ) , β-glucose glycosides enzyme 25 IU/g,xylanase enzyme 120 U/g, PEG6000 2 g/L, enzymatic hydrolysis temperature 50 ℃, and reaction time 36 h, the hydrolysis yield of cellulose and xylan reached 86. 29% and 74. 03%,respectively. They improved 2. 3 times and 2. 8 times of those pretreated by 170 ℃ for 40 min.